Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum

Michael J. Rutten, Robert G. Garrison, C. Diane Moore, A. Max Fiskin, Laurence Y. Cheung

Research output: Contribution to journalArticle

Abstract

Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific localization of alkaline phosphatase activity to the plasma membrane. Treatment of the tissues with levamisole (an alkaline phosphatase inhibitor) did not markedly reduce the specific alkaline phosphatase activity. Specific lead deposition was reduced by removal of the substrate from the reaction mixture. The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis.

Original languageEnglish (US)
Pages (from-to)555-561
Number of pages7
JournalCell and tissue research
Volume258
Issue number3
DOIs
StatePublished - Dec 1 1989
Externally publishedYes

Keywords

  • Alkaline phosphatase
  • Cytochemistry
  • Endoplasmic reticulum, smooth
  • G cell
  • Gastrin
  • Golgi complex
  • Necturus maculosus (Urodela)

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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  • Cite this

    Rutten, M. J., Garrison, R. G., Moore, C. D., Fiskin, A. M., & Cheung, L. Y. (1989). Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum. Cell and tissue research, 258(3), 555-561. https://doi.org/10.1007/BF00218867