@article{8f55a497e5264226a7fbce9395ff80aa,
title = "Electron cryo-tomography of vestibular hair-cell stereocilia",
abstract = "High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.",
keywords = "Actin, Cryo-electron microscopy, Hair cells, Stereocilia",
author = "Zoltan Metlagel and Krey, {Jocelyn F.} and Junha Song and Swift, {Mark F.} and Tivol, {William J.} and Dumont, {Rachel A.} and Jasmine Thai and Alex Chang and Helia Seifikar and Niels Volkmann and Dorit Hanein and Barr-Gillespie, {Peter G.} and Manfred Auer",
note = "Funding Information: MA acknowledges support by National Institutes of Health (NIH) grant P01 GM051487. PGBG was supported by NIH grants R01 DC011034 and P30 DC005983; DH was supported by NIH grant R01 GM115972; and NV was supported by NIH grant P01 GM121203. NIH grants S10 OD012372 (DH) and P01 GM098412-S1 (DH) funded the purchase of the Titan Krios transmission electron microscope and Falcon II direct electron detector used for some data acquisition in this study. We received support from the OHSU Multiscale Microscopy Center, where additional data were acquired using a Titan Krios electron microscope. Funding Information: MA acknowledges support by National Institutes of Health (NIH) grant P01 GM051487 . PGBG was supported by NIH grants R01 DC011034 and P30 DC005983 ; DH was supported by NIH grant R01 GM115972 ; and NV was supported by NIH grant P01 GM121203 . NIH grants S10 OD012372 (DH) and P01 GM098412-S1 (DH) funded the purchase of the Titan Krios transmission electron microscope and Falcon II direct electron detector used for some data acquisition in this study. We received support from the OHSU Multiscale Microscopy Center, where additional data were acquired using a Titan Krios electron microscope. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = may,
day = "1",
doi = "10.1016/j.jsb.2019.02.006",
language = "English (US)",
volume = "206",
pages = "149--155",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "2",
}