Electrokinetic assembly of microsphere and cellular arrays

Mihrimah Ozkan, Sadik Esener, Sangeeta N. Bhatia

Research output: Contribution to journalArticle

Abstract

We have developed a novel electrochemical system for field assisted, fluidic assembly of objects on a microfabricated silicon substrate by means of electrical addressing. The principle of our technique is based on the movement of charged species in solution to oppositely charged electrodes, as seen commonly in electrophoresis. Here, charged species such as beads and cells are moved electrokinetically through an aqueous solution towards a charged electrode. Micro patterning of the electrodes allows localization of charged species. We present a theoretical framework to predict the electric potential for assembly and disassembly of spherical objects. We correlate theoretical predictions with the motion of negatively charged polystyrene beads of 20 μm diameter on 100 μm feature micro patterned substrates. In addition, we extended these results to arraying of 20-30 μm diameter live mammalian cells by means of electrical addressing. This technique has applications in creation of 'active' cellular arrays for cell biology research, drug discovery and tissue engineering.

Original languageEnglish (US)
JournalUnknown Journal
Volume662
StatePublished - 2001
Externally publishedYes

Fingerprint

Cellular arrays
Microspheres
Electrodes
Cytology
Polystyrenes
Fluidics
Silicon
Substrates
Drug Discovery
Tissue Engineering
Electrophoresis
Tissue engineering
Cell Biology
Cells
Electric potential
Research

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

Cite this

Electrokinetic assembly of microsphere and cellular arrays. / Ozkan, Mihrimah; Esener, Sadik; Bhatia, Sangeeta N.

In: Unknown Journal, Vol. 662, 2001.

Research output: Contribution to journalArticle

Ozkan, Mihrimah ; Esener, Sadik ; Bhatia, Sangeeta N. / Electrokinetic assembly of microsphere and cellular arrays. In: Unknown Journal. 2001 ; Vol. 662.
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