Electrical nanowell diagnostics sensors for rapid and ultrasensitive detection of prostate-specific antigen

Anjan Panneer Selvam, Shalini Prasad, Thomas W. Barrett, Steven (Steve) Kazmierczak

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aim: Alumina nanowell based disposable diagnostic biosensor for detecting and quantifying levels of prostate-specific antigen (PSA) from human serum has been designed, fabricated and tested. Materials & methods: The biosensors were designed by integrating nanoporous alumina membranes onto printed circuit board platforms, resulting in the generation of high-density nanowell arrays with gold base electrodes. The size and density of the nanowells were leveraged toward achieving sieving action for size-based exclusion of nonspecific molecules and size-based confinement of the target PSA molecules. Results & conclusion: We demonstrated PSA detection between 0.01 and 1000 ng/ml and detection and quantification of PSA from a 17 patient cohort validated using the Beckman Access system with >95% correlation.

Original languageEnglish (US)
Pages (from-to)2527-2536
Number of pages10
JournalNanomedicine
Volume10
Issue number16
DOIs
StatePublished - Aug 1 2015

Fingerprint

Prostate-Specific Antigen
Antigens
antigen
gold
quantification
diagnostic
exclusion
Aluminum Oxide
Biosensing Techniques
sensor
Biosensors
Sensors
aluminum oxide
Molecules
Alumina
Printed circuit boards
Gold
sieving
Electrodes
Membranes

Keywords

  • alumina nanowells
  • charge screening
  • electrochemical impedance spectroscopy
  • nanowell biosensor diagnostics
  • prostate-specific antigen

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

Cite this

Electrical nanowell diagnostics sensors for rapid and ultrasensitive detection of prostate-specific antigen. / Selvam, Anjan Panneer; Prasad, Shalini; Barrett, Thomas W.; Kazmierczak, Steven (Steve).

In: Nanomedicine, Vol. 10, No. 16, 01.08.2015, p. 2527-2536.

Research output: Contribution to journalArticle

Selvam, Anjan Panneer ; Prasad, Shalini ; Barrett, Thomas W. ; Kazmierczak, Steven (Steve). / Electrical nanowell diagnostics sensors for rapid and ultrasensitive detection of prostate-specific antigen. In: Nanomedicine. 2015 ; Vol. 10, No. 16. pp. 2527-2536.
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