Abstract
Aim: Alumina nanowell based disposable diagnostic biosensor for detecting and quantifying levels of prostate-specific antigen (PSA) from human serum has been designed, fabricated and tested. Materials & methods: The biosensors were designed by integrating nanoporous alumina membranes onto printed circuit board platforms, resulting in the generation of high-density nanowell arrays with gold base electrodes. The size and density of the nanowells were leveraged toward achieving sieving action for size-based exclusion of nonspecific molecules and size-based confinement of the target PSA molecules. Results & conclusion: We demonstrated PSA detection between 0.01 and 1000 ng/ml and detection and quantification of PSA from a 17 patient cohort validated using the Beckman Access system with >95% correlation.
Original language | English (US) |
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Pages (from-to) | 2527-2536 |
Number of pages | 10 |
Journal | Nanomedicine |
Volume | 10 |
Issue number | 16 |
DOIs | |
State | Published - Aug 1 2015 |
Keywords
- alumina nanowells
- charge screening
- electrochemical impedance spectroscopy
- nanowell biosensor diagnostics
- prostate-specific antigen
ASJC Scopus subject areas
- Bioengineering
- Development
- Biomedical Engineering
- General Materials Science
- Medicine (miscellaneous)