Electrical nanowell diagnostics sensors for rapid and ultrasensitive detection of prostate-specific antigen

Anjan Panneer Selvam, Shalini Prasad, Thomas W. Barrett, Steven C. Kazmierczak

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Aim: Alumina nanowell based disposable diagnostic biosensor for detecting and quantifying levels of prostate-specific antigen (PSA) from human serum has been designed, fabricated and tested. Materials & methods: The biosensors were designed by integrating nanoporous alumina membranes onto printed circuit board platforms, resulting in the generation of high-density nanowell arrays with gold base electrodes. The size and density of the nanowells were leveraged toward achieving sieving action for size-based exclusion of nonspecific molecules and size-based confinement of the target PSA molecules. Results & conclusion: We demonstrated PSA detection between 0.01 and 1000 ng/ml and detection and quantification of PSA from a 17 patient cohort validated using the Beckman Access system with >95% correlation.

Original languageEnglish (US)
Pages (from-to)2527-2536
Number of pages10
JournalNanomedicine
Volume10
Issue number16
DOIs
StatePublished - Aug 1 2015

Keywords

  • alumina nanowells
  • charge screening
  • electrochemical impedance spectroscopy
  • nanowell biosensor diagnostics
  • prostate-specific antigen

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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