Electric manipulation of bioparticles and macromolecules on microfabricated electrodes

Y. Huang, K. L. Ewalt, M. Tirado, R. Haigis, A. Forster, D. Ackley, Michael (Mike) Heller, J. P. O'Connell, M. Krihak

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Bioparticle separation, bioparticle enrichment, and electric field-mediated immune detection were carried out on microfabricated semiconductor chips utilizing ac and dc electric fields. Microscale separation on a chip surface having an active area of ∼16 mm2 was demonstrated for a mixture of Bacillus globigii spores and Escherichia coli bacteria. Dielectrophoretic enrichment was performed by collecting target bioparticles from a flow stream in flow cells of ∼7.5 μL, achieving a 20-fold increase in the concentration of E. coli bacteria from a diluted sample, a 28-fold enrichment for peripheral blood mononuclear cells from red blood cells, and a 30-fold increase in white blood cells from diluted whole blood. The ability to manipulate and collect bioparticles and macromolecules at microfabricated electrodes with ac and dc fields was further illustrated in electric field-mediated immunoassays for analyzing the biological identities of E. coli bacteria and B. globigii spores. According to these results, the electric methods for manipulating bioparticles present themselves as viable techniques for novel biomedical applications in sample preparations and biochemical assays on microelectrode arrays.

Original languageEnglish (US)
Pages (from-to)1549-1559
Number of pages11
JournalAnalytical Chemistry
Volume73
Issue number7
DOIs
StatePublished - Apr 1 2001
Externally publishedYes

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Macromolecules
Blood
Escherichia coli
Bacteria
Electrodes
Electric fields
Cells
Microelectrodes
Bacilli
Assays
Semiconductor materials

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Huang, Y., Ewalt, K. L., Tirado, M., Haigis, R., Forster, A., Ackley, D., ... Krihak, M. (2001). Electric manipulation of bioparticles and macromolecules on microfabricated electrodes. Analytical Chemistry, 73(7), 1549-1559. https://doi.org/10.1021/ac001109s

Electric manipulation of bioparticles and macromolecules on microfabricated electrodes. / Huang, Y.; Ewalt, K. L.; Tirado, M.; Haigis, R.; Forster, A.; Ackley, D.; Heller, Michael (Mike); O'Connell, J. P.; Krihak, M.

In: Analytical Chemistry, Vol. 73, No. 7, 01.04.2001, p. 1549-1559.

Research output: Contribution to journalArticle

Huang, Y, Ewalt, KL, Tirado, M, Haigis, R, Forster, A, Ackley, D, Heller, MM, O'Connell, JP & Krihak, M 2001, 'Electric manipulation of bioparticles and macromolecules on microfabricated electrodes', Analytical Chemistry, vol. 73, no. 7, pp. 1549-1559. https://doi.org/10.1021/ac001109s
Huang Y, Ewalt KL, Tirado M, Haigis R, Forster A, Ackley D et al. Electric manipulation of bioparticles and macromolecules on microfabricated electrodes. Analytical Chemistry. 2001 Apr 1;73(7):1549-1559. https://doi.org/10.1021/ac001109s
Huang, Y. ; Ewalt, K. L. ; Tirado, M. ; Haigis, R. ; Forster, A. ; Ackley, D. ; Heller, Michael (Mike) ; O'Connell, J. P. ; Krihak, M. / Electric manipulation of bioparticles and macromolecules on microfabricated electrodes. In: Analytical Chemistry. 2001 ; Vol. 73, No. 7. pp. 1549-1559.
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