Electric manipulation of bioparticles and macromolecules on microfabricated electrodes

Y. Huang, K. L. Ewalt, M. Tirado, R. Haigis, A. Forster, D. Ackley, M. J. Heller, J. P. O'Connell, M. Krihak

Research output: Contribution to journalArticle

151 Scopus citations

Abstract

Bioparticle separation, bioparticle enrichment, and electric field-mediated immune detection were carried out on microfabricated semiconductor chips utilizing ac and dc electric fields. Microscale separation on a chip surface having an active area of ∼16 mm2 was demonstrated for a mixture of Bacillus globigii spores and Escherichia coli bacteria. Dielectrophoretic enrichment was performed by collecting target bioparticles from a flow stream in flow cells of ∼7.5 μL, achieving a 20-fold increase in the concentration of E. coli bacteria from a diluted sample, a 28-fold enrichment for peripheral blood mononuclear cells from red blood cells, and a 30-fold increase in white blood cells from diluted whole blood. The ability to manipulate and collect bioparticles and macromolecules at microfabricated electrodes with ac and dc fields was further illustrated in electric field-mediated immunoassays for analyzing the biological identities of E. coli bacteria and B. globigii spores. According to these results, the electric methods for manipulating bioparticles present themselves as viable techniques for novel biomedical applications in sample preparations and biochemical assays on microelectrode arrays.

Original languageEnglish (US)
Pages (from-to)1549-1559
Number of pages11
JournalAnalytical Chemistry
Volume73
Issue number7
DOIs
StatePublished - Apr 1 2001

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Electric manipulation of bioparticles and macromolecules on microfabricated electrodes'. Together they form a unique fingerprint.

  • Cite this

    Huang, Y., Ewalt, K. L., Tirado, M., Haigis, R., Forster, A., Ackley, D., Heller, M. J., O'Connell, J. P., & Krihak, M. (2001). Electric manipulation of bioparticles and macromolecules on microfabricated electrodes. Analytical Chemistry, 73(7), 1549-1559. https://doi.org/10.1021/ac001109s