Electric-field-directed self-assembly of active enzyme-nanoparticle structures

Alexander P. Hsiao, Michael (Mike) Heller

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A method is presented for the electric-field-directed self-assembly of higher-order structures composed of alternating layers of biotin nanoparticles and streptavidin-/avidin-conjugated enzymes carried out on a microelectrode array device. Enzymes included in the study were glucose oxidase (GOx), horseradish peroxidase (HRP), and alkaline phosphatase (AP); all of which could be used to form a light-emitting microscale glucose sensor. Directed assembly included fabricating multilayer structures with 200nm or 40nm GOx-avidin-biotin nanoparticles, with AP-streptavidin-biotin nanoparticles, and with HRP-streptavidin-biotin nanoparticles. Multilayered structures were also fabricated with alternate layering of HRP-streptavidin-biotin nanoparticles and GOx-avidin-biotin nanoparticles. Results showed that enzymatic activity was retained after the assembly process, indicating that substrates could still diffuse into the structures and that the electric-field-based fabrication process itself did not cause any significant loss of enzyme activity. These methods provide a solution to overcome the cumbersome passive layer-by-layer assembly methods to efficiently fabricate higher-order active biological and chemical hybrid structures that can be useful for creating novel biosensors and drug delivery nanostructures, as well as for diagnostic applications.

Original languageEnglish (US)
Article number178487
JournalJournal of Biomedicine and Biotechnology
Volume2012
DOIs
StatePublished - Mar 16 2012
Externally publishedYes

Fingerprint

Biotin
Nanoparticles
Self assembly
Streptavidin
Electric fields
Glucose Oxidase
Avidin
Enzymes
Horseradish Peroxidase
Alkaline Phosphatase
Glucose sensors
Nanostructures
Microelectrodes
Enzyme activity
Biosensing Techniques
Drug delivery
Biosensors
Multilayers
Light
Glucose

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

Electric-field-directed self-assembly of active enzyme-nanoparticle structures. / Hsiao, Alexander P.; Heller, Michael (Mike).

In: Journal of Biomedicine and Biotechnology, Vol. 2012, 178487, 16.03.2012.

Research output: Contribution to journalArticle

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