Efficient coupling of transducin to monomeric rhodopsin in a phospholipid bilayer

Matthew Whorton, Beata Jastrzebska, Paul S H Park, Dimitrios Fotiadis, Andreas Engel, Krzysztof Palczewski, Roger K. Sunahara

Research output: Contribution to journalArticle

203 Citations (Scopus)

Abstract

G protein-coupled receptors (GPCRs) are seven transmembrane domain proteins that transduce extracellular signals across the plasma membrane and couple to the heterotrimeric family of G proteins. Like most intrinsic membrane proteins, GPCRs are capable of oligomerization, the function of which has only been established for a few different receptor systems. One challenge in understanding the function of oligomers relates to the inability to separate monomeric and oligomeric receptor complexes in membrane environments. Here we report the reconstitution of bovine rhodopsin, a GPCR expressed in the retina, into an apolipoprotein A-I phospholipid particle, derived from high density lipoprotein (HDL). We demonstrate that rhodopsin, when incorporated into these 10 nm reconstituted HDL (rHDL) particles, is monomeric and functional. Rhodopsin·rHDL maintains the appropriate spectral properties with respect to photoactivation and formation of the active form, metarhodopsin II. Additionally, the kinetics of metarhodopsin II decay is similar between rhodopsin in native membranes and rhodopsin in rHDL particles. Photoactivation of monomeric rhodopsin·rHDL also results in the rapid activation of transducin, at a rate that is comparable with that found in native rod outer segments and 20-fold faster than rhodopsin in detergent micelles. These data suggest that monomeric rhodopsin is the minimal functional unit in G protein activation and that oligomerization is not absolutely required for this process.

Original languageEnglish (US)
Pages (from-to)4387-4394
Number of pages8
JournalJournal of Biological Chemistry
Volume283
Issue number7
DOIs
StatePublished - Feb 15 2008
Externally publishedYes

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Transducin
Rhodopsin
Phospholipids
Oligomerization
HDL Lipoproteins
G-Protein-Coupled Receptors
GTP-Binding Proteins
Chemical activation
Membranes
Rod Cell Outer Segment
Heterotrimeric GTP-Binding Proteins
Apolipoprotein A-I
Micelles
Cell membranes
Oligomers
Detergents
Retina
Membrane Proteins
Cell Membrane
Kinetics

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Whorton, M., Jastrzebska, B., Park, P. S. H., Fotiadis, D., Engel, A., Palczewski, K., & Sunahara, R. K. (2008). Efficient coupling of transducin to monomeric rhodopsin in a phospholipid bilayer. Journal of Biological Chemistry, 283(7), 4387-4394. https://doi.org/10.1074/jbc.M703346200

Efficient coupling of transducin to monomeric rhodopsin in a phospholipid bilayer. / Whorton, Matthew; Jastrzebska, Beata; Park, Paul S H; Fotiadis, Dimitrios; Engel, Andreas; Palczewski, Krzysztof; Sunahara, Roger K.

In: Journal of Biological Chemistry, Vol. 283, No. 7, 15.02.2008, p. 4387-4394.

Research output: Contribution to journalArticle

Whorton, M, Jastrzebska, B, Park, PSH, Fotiadis, D, Engel, A, Palczewski, K & Sunahara, RK 2008, 'Efficient coupling of transducin to monomeric rhodopsin in a phospholipid bilayer', Journal of Biological Chemistry, vol. 283, no. 7, pp. 4387-4394. https://doi.org/10.1074/jbc.M703346200
Whorton, Matthew ; Jastrzebska, Beata ; Park, Paul S H ; Fotiadis, Dimitrios ; Engel, Andreas ; Palczewski, Krzysztof ; Sunahara, Roger K. / Efficient coupling of transducin to monomeric rhodopsin in a phospholipid bilayer. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 7. pp. 4387-4394.
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