Efficient Bacterial Expression of Bovine and Porcine Growth Hormones

Peter H. Seeburg; Stacey Sias; John Adelman; Herman A. de Boer; Joel Hayflick; Parkash Jhurani; David V. Goeddel; Herbert L. Heyneker

doi:10.1089/dna.1.1983.2.37

Efficient Bacterial Expression of Bovine and Porcine Growth Hormones

Peter H. Seeburg, Stacey Sias, John Adelman, Herman A. de Boer, Joel Hayflick, Parkash Jhurani, David V. Goeddel, Herbert L. Heyneker

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

cDNAs prepared using poly(A)mRNA from pituitaries and containing the coding sequences for bovine and porcine growth hormones (bGH and pGH) were cloned in bacteria. The primary structures of the peptide hormones derived from the nucleotide sequences of the respective cDNAs show approximately 90% homology. The cloned cDNAs were modified using synthetic DNA to construct expression vectors for efficient bacterial production of the mature animal growth hormones.

Original language	English (US)
Pages (from-to)	37-45
Number of pages	9
Journal	DNA
Volume	2
Issue number	1
DOIs	https://doi.org/10.1089/dna.1.1983.2.37
State	Published - 1983
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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10.1089/dna.1.1983.2.37

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author = "Seeburg, {Peter H.} and Stacey Sias and John Adelman and {de Boer}, {Herman A.} and Joel Hayflick and Parkash Jhurani and Goeddel, {David V.} and Heyneker, {Herbert L.}",

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doi = "10.1089/dna.1.1983.2.37",

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AU - Seeburg, Peter H.

AU - Sias, Stacey

AU - Adelman, John

AU - de Boer, Herman A.

AU - Hayflick, Joel

AU - Jhurani, Parkash

AU - Goeddel, David V.

AU - Heyneker, Herbert L.

PY - 1983

Y1 - 1983

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AB - cDNAs prepared using poly(A)mRNA from pituitaries and containing the coding sequences for bovine and porcine growth hormones (bGH and pGH) were cloned in bacteria. The primary structures of the peptide hormones derived from the nucleotide sequences of the respective cDNAs show approximately 90% homology. The cloned cDNAs were modified using synthetic DNA to construct expression vectors for efficient bacterial production of the mature animal growth hormones.

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Efficient Bacterial Expression of Bovine and Porcine Growth Hormones

Abstract

ASJC Scopus subject areas

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