Efficient Bacterial Expression of Bovine and Porcine Growth Hormones

Peter H. Seeburg, Stacey Sias, John Adelman, Herman A. de Boer, Joel Hayflick, Parkash Jhurani, David V. Goeddel, Herbert L. Heyneker

Research output: Contribution to journalArticle

121 Scopus citations

Abstract

cDNAs prepared using poly(A)mRNA from pituitaries and containing the coding sequences for bovine and porcine growth hormones (bGH and pGH) were cloned in bacteria. The primary structures of the peptide hormones derived from the nucleotide sequences of the respective cDNAs show approximately 90% homology. The cloned cDNAs were modified using synthetic DNA to construct expression vectors for efficient bacterial production of the mature animal growth hormones.

Original languageEnglish (US)
Pages (from-to)37-45
Number of pages9
JournalDNA
Volume2
Issue number1
DOIs
StatePublished - Jan 1 1983

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Seeburg, P. H., Sias, S., Adelman, J., de Boer, H. A., Hayflick, J., Jhurani, P., Goeddel, D. V., & Heyneker, H. L. (1983). Efficient Bacterial Expression of Bovine and Porcine Growth Hormones. DNA, 2(1), 37-45. https://doi.org/10.1089/dna.1.1983.2.37