Efficient bacterial expression of bovine and porcine growth hormones

P. H. Seeburg, S. Sias, John Adelman, H. A. de Boer, J. Hayflick, P. Jhurani, D. V. Goeddel, H. L. Heyneker

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

cDNAs prepared using poly(A)mRNA from pituitaries and containing the coding sequences for bovine and porcine growth hormones (bGH and pGH) were cloned in bacteria. The primary structures ot the peptide hormones derived from the nucleotide sequences of the respective cDNAs show approximately 90% homology. The cloned cDNAs were modified using synthetic DNA to construct expression vectors for efficient bacterial production of the mature animal growth hormones.

Original languageEnglish (US)
Pages (from-to)37-45
Number of pages9
JournalDNA
Volume2
Issue number1
StatePublished - 1983
Externally publishedYes

Fingerprint

Growth Hormone
Swine
Complementary DNA
Peptide Hormones
Bacteria
Animals
Nucleotides
Messenger RNA
DNA
bovine growth hormone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Seeburg, P. H., Sias, S., Adelman, J., de Boer, H. A., Hayflick, J., Jhurani, P., ... Heyneker, H. L. (1983). Efficient bacterial expression of bovine and porcine growth hormones. DNA, 2(1), 37-45.

Efficient bacterial expression of bovine and porcine growth hormones. / Seeburg, P. H.; Sias, S.; Adelman, John; de Boer, H. A.; Hayflick, J.; Jhurani, P.; Goeddel, D. V.; Heyneker, H. L.

In: DNA, Vol. 2, No. 1, 1983, p. 37-45.

Research output: Contribution to journalArticle

Seeburg, PH, Sias, S, Adelman, J, de Boer, HA, Hayflick, J, Jhurani, P, Goeddel, DV & Heyneker, HL 1983, 'Efficient bacterial expression of bovine and porcine growth hormones', DNA, vol. 2, no. 1, pp. 37-45.
Seeburg PH, Sias S, Adelman J, de Boer HA, Hayflick J, Jhurani P et al. Efficient bacterial expression of bovine and porcine growth hormones. DNA. 1983;2(1):37-45.
Seeburg, P. H. ; Sias, S. ; Adelman, John ; de Boer, H. A. ; Hayflick, J. ; Jhurani, P. ; Goeddel, D. V. ; Heyneker, H. L. / Efficient bacterial expression of bovine and porcine growth hormones. In: DNA. 1983 ; Vol. 2, No. 1. pp. 37-45.
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