Effects of the lazaroid U74389G (21 aminosteroid) on skeletal muscle reperfusion injury in rabbits

J. J. Hoballah, C. R. Mohan, Paul Schipper, R. T. Chalmers, J. D. Corson

Research output: Contribution to journalArticle

Abstract

The purpose of this experiment was to evaluate the effects of the aminosteroid U74389G on skeletal muscle reperfusion injury in rabbits. In 24 white New Zealand rabbits (weighing 7.0-8.0 lb), the rectus femoris muscle on both sides was completely isolated on a single vascular pedicle (artery and vein) and a major accessory vein. All muscles were weighed using a suspension spring balance and then underwent 4 hours of normothermic ischemia followed by 24 hours of reperfusion. Muscle ischemia was induced by the application of atraumatic vascular clamps to the vascular pedicles. Complete muscle ischemia and reperfusion were documented by a laser flow meter. The animals were divided into three groups; Group I (n = 8) served as control, Group II (n = 8) received an IV bolus of U74389G (1.5 mg/kg) five minutes prior to ischemia, Group III (n = 8) was given the same dose of lazaroid five minutes prior to reperfusion. Muscle biopsies were obtained before ischemia and after reperfusion for quantification of myeloperoxidase (MPO) activity. At the completion of reperfusion, the muscles were excised, weighed and cut into slices along the longitudinal axis and then incubated for 30 minutes in 0.05% nitroblue tetrazolium. Areas of necrosis were determined by computerized planimetry. The following results indicate that reperfusion muscle necrosis in rabbits is significantly decreased by the administration of the lazaroid U74389G. Leukocyte sequestration was not affected by the lazaroid administration. These beneficial effects were observed whether the lazaroid was administered prior to ischemia or prior to reperfusion and were independent of leukocyte sequestration.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalInternational Angiology
Volume15
Issue number1
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

Reperfusion Injury
Reperfusion
Skeletal Muscle
Rabbits
Ischemia
Muscles
Blood Vessels
Veins
Leukocytes
Necrosis
Nitroblue Tetrazolium
Quadriceps Muscle
Peroxidase
Suspensions
Lasers
Arteries
Biopsy
Control Groups

Keywords

  • 21 aminosteroids
  • Ischemia reperfusion
  • Lazaroids
  • Skeletal muscle

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Hoballah, J. J., Mohan, C. R., Schipper, P., Chalmers, R. T., & Corson, J. D. (1996). Effects of the lazaroid U74389G (21 aminosteroid) on skeletal muscle reperfusion injury in rabbits. International Angiology, 15(1), 61-66.

Effects of the lazaroid U74389G (21 aminosteroid) on skeletal muscle reperfusion injury in rabbits. / Hoballah, J. J.; Mohan, C. R.; Schipper, Paul; Chalmers, R. T.; Corson, J. D.

In: International Angiology, Vol. 15, No. 1, 03.1996, p. 61-66.

Research output: Contribution to journalArticle

Hoballah, J. J. ; Mohan, C. R. ; Schipper, Paul ; Chalmers, R. T. ; Corson, J. D. / Effects of the lazaroid U74389G (21 aminosteroid) on skeletal muscle reperfusion injury in rabbits. In: International Angiology. 1996 ; Vol. 15, No. 1. pp. 61-66.
@article{48fe5c9d1bf545b598964ab4922148f4,
title = "Effects of the lazaroid U74389G (21 aminosteroid) on skeletal muscle reperfusion injury in rabbits",
abstract = "The purpose of this experiment was to evaluate the effects of the aminosteroid U74389G on skeletal muscle reperfusion injury in rabbits. In 24 white New Zealand rabbits (weighing 7.0-8.0 lb), the rectus femoris muscle on both sides was completely isolated on a single vascular pedicle (artery and vein) and a major accessory vein. All muscles were weighed using a suspension spring balance and then underwent 4 hours of normothermic ischemia followed by 24 hours of reperfusion. Muscle ischemia was induced by the application of atraumatic vascular clamps to the vascular pedicles. Complete muscle ischemia and reperfusion were documented by a laser flow meter. The animals were divided into three groups; Group I (n = 8) served as control, Group II (n = 8) received an IV bolus of U74389G (1.5 mg/kg) five minutes prior to ischemia, Group III (n = 8) was given the same dose of lazaroid five minutes prior to reperfusion. Muscle biopsies were obtained before ischemia and after reperfusion for quantification of myeloperoxidase (MPO) activity. At the completion of reperfusion, the muscles were excised, weighed and cut into slices along the longitudinal axis and then incubated for 30 minutes in 0.05{\%} nitroblue tetrazolium. Areas of necrosis were determined by computerized planimetry. The following results indicate that reperfusion muscle necrosis in rabbits is significantly decreased by the administration of the lazaroid U74389G. Leukocyte sequestration was not affected by the lazaroid administration. These beneficial effects were observed whether the lazaroid was administered prior to ischemia or prior to reperfusion and were independent of leukocyte sequestration.",
keywords = "21 aminosteroids, Ischemia reperfusion, Lazaroids, Skeletal muscle",
author = "Hoballah, {J. J.} and Mohan, {C. R.} and Paul Schipper and Chalmers, {R. T.} and Corson, {J. D.}",
year = "1996",
month = "3",
language = "English (US)",
volume = "15",
pages = "61--66",
journal = "International Angiology",
issn = "0392-9590",
publisher = "Edizioni Minerva Medica S.p.A.",
number = "1",

}

TY - JOUR

T1 - Effects of the lazaroid U74389G (21 aminosteroid) on skeletal muscle reperfusion injury in rabbits

AU - Hoballah, J. J.

AU - Mohan, C. R.

AU - Schipper, Paul

AU - Chalmers, R. T.

AU - Corson, J. D.

PY - 1996/3

Y1 - 1996/3

N2 - The purpose of this experiment was to evaluate the effects of the aminosteroid U74389G on skeletal muscle reperfusion injury in rabbits. In 24 white New Zealand rabbits (weighing 7.0-8.0 lb), the rectus femoris muscle on both sides was completely isolated on a single vascular pedicle (artery and vein) and a major accessory vein. All muscles were weighed using a suspension spring balance and then underwent 4 hours of normothermic ischemia followed by 24 hours of reperfusion. Muscle ischemia was induced by the application of atraumatic vascular clamps to the vascular pedicles. Complete muscle ischemia and reperfusion were documented by a laser flow meter. The animals were divided into three groups; Group I (n = 8) served as control, Group II (n = 8) received an IV bolus of U74389G (1.5 mg/kg) five minutes prior to ischemia, Group III (n = 8) was given the same dose of lazaroid five minutes prior to reperfusion. Muscle biopsies were obtained before ischemia and after reperfusion for quantification of myeloperoxidase (MPO) activity. At the completion of reperfusion, the muscles were excised, weighed and cut into slices along the longitudinal axis and then incubated for 30 minutes in 0.05% nitroblue tetrazolium. Areas of necrosis were determined by computerized planimetry. The following results indicate that reperfusion muscle necrosis in rabbits is significantly decreased by the administration of the lazaroid U74389G. Leukocyte sequestration was not affected by the lazaroid administration. These beneficial effects were observed whether the lazaroid was administered prior to ischemia or prior to reperfusion and were independent of leukocyte sequestration.

AB - The purpose of this experiment was to evaluate the effects of the aminosteroid U74389G on skeletal muscle reperfusion injury in rabbits. In 24 white New Zealand rabbits (weighing 7.0-8.0 lb), the rectus femoris muscle on both sides was completely isolated on a single vascular pedicle (artery and vein) and a major accessory vein. All muscles were weighed using a suspension spring balance and then underwent 4 hours of normothermic ischemia followed by 24 hours of reperfusion. Muscle ischemia was induced by the application of atraumatic vascular clamps to the vascular pedicles. Complete muscle ischemia and reperfusion were documented by a laser flow meter. The animals were divided into three groups; Group I (n = 8) served as control, Group II (n = 8) received an IV bolus of U74389G (1.5 mg/kg) five minutes prior to ischemia, Group III (n = 8) was given the same dose of lazaroid five minutes prior to reperfusion. Muscle biopsies were obtained before ischemia and after reperfusion for quantification of myeloperoxidase (MPO) activity. At the completion of reperfusion, the muscles were excised, weighed and cut into slices along the longitudinal axis and then incubated for 30 minutes in 0.05% nitroblue tetrazolium. Areas of necrosis were determined by computerized planimetry. The following results indicate that reperfusion muscle necrosis in rabbits is significantly decreased by the administration of the lazaroid U74389G. Leukocyte sequestration was not affected by the lazaroid administration. These beneficial effects were observed whether the lazaroid was administered prior to ischemia or prior to reperfusion and were independent of leukocyte sequestration.

KW - 21 aminosteroids

KW - Ischemia reperfusion

KW - Lazaroids

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=0029868013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029868013&partnerID=8YFLogxK

M3 - Article

C2 - 8739539

AN - SCOPUS:0029868013

VL - 15

SP - 61

EP - 66

JO - International Angiology

JF - International Angiology

SN - 0392-9590

IS - 1

ER -