Effects of the intensity of the suckling stimulus and ovarian steroids on pituitary gonadotropin-releasing hormone receptors during lactation

M (Susan) Smith

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

These studies examined whether the decrease in pituitary responsiveness to gonadotropin-releasing hormone (GnRH) observed during lactation in the rat results from a change in pituitary GnRH receptors. GnRH binding capacity was determined by saturation analysis using D-Ala6 as both ligand and tracer. During the estrous cycle, the number of GnRH binding sites increased from 199 ± 38 fmol/mg protein on estrus to 527 ± 31 fmol/mg protein on the morning of proestrus, whereas there was no change in receptor affinity (K(a), 6-10 x 109 M-1). During lactation, females nursing 8 pups on Days 5 or 10 postpartum had 50% fewer GnRH receptors (109-120 fmol/mg protein) than observed during estrus or diestrus 1 (199-242 fmol/mg protein) although receptor affinity was similar among all the groups. No deficits in pituitary GnRH receptors were observed in females nursing 2 pups on Day 10 postpartum. Removal of the 8-pup suckling stimulus for 24 or 48 h resulted in a dramatic increase in GnRH receptor capacity by 24 h from 120 ± 16 to 355 ± 39 fmol/mg protein. The rise in GnRH receptors after pup removal was accompanied by an increase in serum luteinizing hormone (LH) and estradiol concentrations. To assess the role of ovarian steroids in determining GnRH receptor capacity during lactation, females were ovariectomized (OVX) on Day 2 postpartum. Suckling of a large litter (8 pups) completely blocked the postcastration rise in serum LH and in pituitary GnRH receptors on Day 10 postpartum (OVS+ 8, 77 ± 12 fmol/mg protein; OVX+ 0, 442 ± 38 fmol/mg protein). Furthermore, removal of the 8-pup litters for 24 h from the ovariectomized females produced a dramatic rise in GnRH receptors which was identical to the increase observed in intact females deprived of their litters. These results demonstrate that the suckling stimulus suppresses the number of GnRH binding sires but has no effect on receptor affinity, and the degree of suppression is directly related to the intensity of the suckling stimulus. The large decrease in pituitary GnRH receptor content in the presence of a large sucling stimulus is most likely due to a very dramatic suppression of GnRH release from the hypothalamus.

Original languageEnglish (US)
Pages (from-to)548-555
Number of pages8
JournalBiology of Reproduction
Volume31
Issue number3
StatePublished - 1984
Externally publishedYes

Fingerprint

LHRH Receptors
Pituitary Gonadotropins
Pituitary Hormones
Lactation
Steroids
Gonadotropin-Releasing Hormone
Postpartum Period
Proteins
Estrus
Luteinizing Hormone
Nursing
Diestrus
Proestrus
Estrous Cycle
Serum
Hypothalamus
Estradiol
Binding Sites
Ligands

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Effects of the intensity of the suckling stimulus and ovarian steroids on pituitary gonadotropin-releasing hormone receptors during lactation. / Smith, M (Susan).

In: Biology of Reproduction, Vol. 31, No. 3, 1984, p. 548-555.

Research output: Contribution to journalArticle

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