Effects of taxol on the growth of normal, polyposis, and cancerous human colonic epithelial cells

Brett Sheppard, C. L. Meichsner, Michael Rutten, J. Land, K. A. Bacon, R. A. Crass, Donald Trunkey, Karen Deveney, Clifford Deveney

Research output: Contribution to journalArticle

Abstract

At the present time the effective Taxol dose and time-course for the treatment of colonic cancer cells without disruption of normal colonic cell proliferation is not known. The aim of the present study was to determine the effects of Taxol on the growth of normal, polyposis, and cancerous human colonic cells. Cultures of normal human colonic cells, polyposis cells, colon cancer cells (Caco-2, T-84, LoVo cell lines) were plated in multiwell plates. Cell counts or MTT-assay were used to evaluate cell proliferation. Immunofluorescence and Western-blotting measured P- glycoprotein expression. Taxol (1×10-9-1×10-8M) was slightly inhibitory but very inhibitory (>10nM) to normal colonic proliferation. Taxol (1×10-9-1×10-8 M) was effective in the inhibition of polyposis and cancerous cell growth but not at higher doses (1×10-8-1×10-6M). Immunofluorescence and Western- immunoblotting showed a direct relationship between Taxol-induced P-glycoprotein expression and the resistance to Taxol in all cell types. We conclude: 1) lower doses of Taxol are more effective in preventing the growth of colon polyposis and cancer cells with minimal effects on the proliferation of normal colon cells; 2) a direct relationship exists between P-glycoprotein expression and Taxol-drug resistance.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

paclitaxel
Paclitaxel
epithelial cells
Epithelial Cells
Growth
P-Glycoprotein
Colonic Neoplasms
Cells
Cell proliferation
cells
fluorescent antibody technique
colon
Fluorescent Antibody Technique
cell proliferation
dosage
Western Blotting
Cell Proliferation
Caco-2 Cells
drug resistance
Cell growth

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Effects of taxol on the growth of normal, polyposis, and cancerous human colonic epithelial cells. / Sheppard, Brett; Meichsner, C. L.; Rutten, Michael; Land, J.; Bacon, K. A.; Crass, R. A.; Trunkey, Donald; Deveney, Karen; Deveney, Clifford.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Sheppard B, Meichsner CL, Rutten M, Land J, Bacon KA, Crass RA et al. Effects of taxol on the growth of normal, polyposis, and cancerous human colonic epithelial cells. FASEB Journal. 1998 Mar 20;12(5).
@article{a4d652d7a7aa4f0990d51602c2ccafcb,
title = "Effects of taxol on the growth of normal, polyposis, and cancerous human colonic epithelial cells",
abstract = "At the present time the effective Taxol dose and time-course for the treatment of colonic cancer cells without disruption of normal colonic cell proliferation is not known. The aim of the present study was to determine the effects of Taxol on the growth of normal, polyposis, and cancerous human colonic cells. Cultures of normal human colonic cells, polyposis cells, colon cancer cells (Caco-2, T-84, LoVo cell lines) were plated in multiwell plates. Cell counts or MTT-assay were used to evaluate cell proliferation. Immunofluorescence and Western-blotting measured P- glycoprotein expression. Taxol (1×10-9-1×10-8M) was slightly inhibitory but very inhibitory (>10nM) to normal colonic proliferation. Taxol (1×10-9-1×10-8 M) was effective in the inhibition of polyposis and cancerous cell growth but not at higher doses (1×10-8-1×10-6M). Immunofluorescence and Western- immunoblotting showed a direct relationship between Taxol-induced P-glycoprotein expression and the resistance to Taxol in all cell types. We conclude: 1) lower doses of Taxol are more effective in preventing the growth of colon polyposis and cancer cells with minimal effects on the proliferation of normal colon cells; 2) a direct relationship exists between P-glycoprotein expression and Taxol-drug resistance.",
author = "Brett Sheppard and Meichsner, {C. L.} and Michael Rutten and J. Land and Bacon, {K. A.} and Crass, {R. A.} and Donald Trunkey and Karen Deveney and Clifford Deveney",
year = "1998",
month = "3",
day = "20",
language = "English (US)",
volume = "12",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "5",

}

TY - JOUR

T1 - Effects of taxol on the growth of normal, polyposis, and cancerous human colonic epithelial cells

AU - Sheppard, Brett

AU - Meichsner, C. L.

AU - Rutten, Michael

AU - Land, J.

AU - Bacon, K. A.

AU - Crass, R. A.

AU - Trunkey, Donald

AU - Deveney, Karen

AU - Deveney, Clifford

PY - 1998/3/20

Y1 - 1998/3/20

N2 - At the present time the effective Taxol dose and time-course for the treatment of colonic cancer cells without disruption of normal colonic cell proliferation is not known. The aim of the present study was to determine the effects of Taxol on the growth of normal, polyposis, and cancerous human colonic cells. Cultures of normal human colonic cells, polyposis cells, colon cancer cells (Caco-2, T-84, LoVo cell lines) were plated in multiwell plates. Cell counts or MTT-assay were used to evaluate cell proliferation. Immunofluorescence and Western-blotting measured P- glycoprotein expression. Taxol (1×10-9-1×10-8M) was slightly inhibitory but very inhibitory (>10nM) to normal colonic proliferation. Taxol (1×10-9-1×10-8 M) was effective in the inhibition of polyposis and cancerous cell growth but not at higher doses (1×10-8-1×10-6M). Immunofluorescence and Western- immunoblotting showed a direct relationship between Taxol-induced P-glycoprotein expression and the resistance to Taxol in all cell types. We conclude: 1) lower doses of Taxol are more effective in preventing the growth of colon polyposis and cancer cells with minimal effects on the proliferation of normal colon cells; 2) a direct relationship exists between P-glycoprotein expression and Taxol-drug resistance.

AB - At the present time the effective Taxol dose and time-course for the treatment of colonic cancer cells without disruption of normal colonic cell proliferation is not known. The aim of the present study was to determine the effects of Taxol on the growth of normal, polyposis, and cancerous human colonic cells. Cultures of normal human colonic cells, polyposis cells, colon cancer cells (Caco-2, T-84, LoVo cell lines) were plated in multiwell plates. Cell counts or MTT-assay were used to evaluate cell proliferation. Immunofluorescence and Western-blotting measured P- glycoprotein expression. Taxol (1×10-9-1×10-8M) was slightly inhibitory but very inhibitory (>10nM) to normal colonic proliferation. Taxol (1×10-9-1×10-8 M) was effective in the inhibition of polyposis and cancerous cell growth but not at higher doses (1×10-8-1×10-6M). Immunofluorescence and Western- immunoblotting showed a direct relationship between Taxol-induced P-glycoprotein expression and the resistance to Taxol in all cell types. We conclude: 1) lower doses of Taxol are more effective in preventing the growth of colon polyposis and cancer cells with minimal effects on the proliferation of normal colon cells; 2) a direct relationship exists between P-glycoprotein expression and Taxol-drug resistance.

UR - http://www.scopus.com/inward/record.url?scp=33749191635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749191635&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749191635

VL - 12

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 5

ER -