Effects of seasons and light therapy on G protein levels in mononuclear leukocytes of patients with seasonal affective disorder

Sofia Avissar, Gabriel Schreiber, Yakov Nechamkin, Isaac Neuhaus, Gregory K. Lam, Paul Schwartz, Erick Turner, Jeffery Matthews, Susana Naim, Norman E. Rosenthal

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Background: Information-transducing heterotrimeric G proteins have been implicated previously in the mechanism of action of mood stabilizers and in the pathophysiology of mood disorders, Mononuclear leukocytes of patients with unipolar and bipolar depression have been characterized by reduced measures of the stimulatory and inhibitory G proteins. In this study, patients with seasonal affective disorder (SAD) were measured for mononuclear leukocyte G protein levels while depressed during the winter, following light therapy, and in remission during the summer. Methods: Twenty-six patients with SAD and 28 healthy subjects were assessed in the study. The immunoreactivities of G5α, G1α, and Gβ subunit proteins were determined by Western blot analysis of mononuclear leukocyte membranes with selective polyclonal antibodies for the various G subunit proteins, followed by densitometric quantitation using an image analysis system. Results: Untreated patients with SAD and winter, atypical-type depression showed significantly reduced mononuclear leukocyte immunoreactive levels of G(s)α and G(i)α proteins, similar to previous observations in patients with nonseasonal major depression. The reduced G protein levels were normalized with 2 weeks of light therapy. The same patients while in remission during the summer had G protein levels that were similar to those of healthy subjects. Conclusions: G protein-immunoreactive measures in patients with SAD are suggested as a state marker for winter depression, which is normalized by light treatment and during the summer. We speculate that light may exert its effects via normalization of transducing (G1 protein) levels, which are thought to be reduced in winter depression.

Original languageEnglish (US)
Pages (from-to)178-183
Number of pages6
JournalArchives of General Psychiatry
Volume56
Issue number2
DOIs
StatePublished - Feb 1999
Externally publishedYes

Fingerprint

Seasonal Affective Disorder
Mononuclear Leukocytes
Phototherapy
GTP-Binding Proteins
Depression
Protein Subunits
Healthy Volunteers
Light
Heterotrimeric GTP-Binding Proteins
Depressive Disorder
Mood Disorders
Bipolar Disorder
Proteins
Western Blotting
Membranes
Antibodies

ASJC Scopus subject areas

  • Psychiatry and Mental health

Cite this

Effects of seasons and light therapy on G protein levels in mononuclear leukocytes of patients with seasonal affective disorder. / Avissar, Sofia; Schreiber, Gabriel; Nechamkin, Yakov; Neuhaus, Isaac; Lam, Gregory K.; Schwartz, Paul; Turner, Erick; Matthews, Jeffery; Naim, Susana; Rosenthal, Norman E.

In: Archives of General Psychiatry, Vol. 56, No. 2, 02.1999, p. 178-183.

Research output: Contribution to journalArticle

Avissar, S, Schreiber, G, Nechamkin, Y, Neuhaus, I, Lam, GK, Schwartz, P, Turner, E, Matthews, J, Naim, S & Rosenthal, NE 1999, 'Effects of seasons and light therapy on G protein levels in mononuclear leukocytes of patients with seasonal affective disorder', Archives of General Psychiatry, vol. 56, no. 2, pp. 178-183. https://doi.org/10.1001/archpsyc.56.2.178
Avissar, Sofia ; Schreiber, Gabriel ; Nechamkin, Yakov ; Neuhaus, Isaac ; Lam, Gregory K. ; Schwartz, Paul ; Turner, Erick ; Matthews, Jeffery ; Naim, Susana ; Rosenthal, Norman E. / Effects of seasons and light therapy on G protein levels in mononuclear leukocytes of patients with seasonal affective disorder. In: Archives of General Psychiatry. 1999 ; Vol. 56, No. 2. pp. 178-183.
@article{82083fe03fdb41f7ad3a8dacd67728c0,
title = "Effects of seasons and light therapy on G protein levels in mononuclear leukocytes of patients with seasonal affective disorder",
abstract = "Background: Information-transducing heterotrimeric G proteins have been implicated previously in the mechanism of action of mood stabilizers and in the pathophysiology of mood disorders, Mononuclear leukocytes of patients with unipolar and bipolar depression have been characterized by reduced measures of the stimulatory and inhibitory G proteins. In this study, patients with seasonal affective disorder (SAD) were measured for mononuclear leukocyte G protein levels while depressed during the winter, following light therapy, and in remission during the summer. Methods: Twenty-six patients with SAD and 28 healthy subjects were assessed in the study. The immunoreactivities of G5α, G1α, and Gβ subunit proteins were determined by Western blot analysis of mononuclear leukocyte membranes with selective polyclonal antibodies for the various G subunit proteins, followed by densitometric quantitation using an image analysis system. Results: Untreated patients with SAD and winter, atypical-type depression showed significantly reduced mononuclear leukocyte immunoreactive levels of G(s)α and G(i)α proteins, similar to previous observations in patients with nonseasonal major depression. The reduced G protein levels were normalized with 2 weeks of light therapy. The same patients while in remission during the summer had G protein levels that were similar to those of healthy subjects. Conclusions: G protein-immunoreactive measures in patients with SAD are suggested as a state marker for winter depression, which is normalized by light treatment and during the summer. We speculate that light may exert its effects via normalization of transducing (G1 protein) levels, which are thought to be reduced in winter depression.",
author = "Sofia Avissar and Gabriel Schreiber and Yakov Nechamkin and Isaac Neuhaus and Lam, {Gregory K.} and Paul Schwartz and Erick Turner and Jeffery Matthews and Susana Naim and Rosenthal, {Norman E.}",
year = "1999",
month = "2",
doi = "10.1001/archpsyc.56.2.178",
language = "English (US)",
volume = "56",
pages = "178--183",
journal = "JAMA Psychiatry",
issn = "2168-622X",
publisher = "American Medical Association",
number = "2",

}

TY - JOUR

T1 - Effects of seasons and light therapy on G protein levels in mononuclear leukocytes of patients with seasonal affective disorder

AU - Avissar, Sofia

AU - Schreiber, Gabriel

AU - Nechamkin, Yakov

AU - Neuhaus, Isaac

AU - Lam, Gregory K.

AU - Schwartz, Paul

AU - Turner, Erick

AU - Matthews, Jeffery

AU - Naim, Susana

AU - Rosenthal, Norman E.

PY - 1999/2

Y1 - 1999/2

N2 - Background: Information-transducing heterotrimeric G proteins have been implicated previously in the mechanism of action of mood stabilizers and in the pathophysiology of mood disorders, Mononuclear leukocytes of patients with unipolar and bipolar depression have been characterized by reduced measures of the stimulatory and inhibitory G proteins. In this study, patients with seasonal affective disorder (SAD) were measured for mononuclear leukocyte G protein levels while depressed during the winter, following light therapy, and in remission during the summer. Methods: Twenty-six patients with SAD and 28 healthy subjects were assessed in the study. The immunoreactivities of G5α, G1α, and Gβ subunit proteins were determined by Western blot analysis of mononuclear leukocyte membranes with selective polyclonal antibodies for the various G subunit proteins, followed by densitometric quantitation using an image analysis system. Results: Untreated patients with SAD and winter, atypical-type depression showed significantly reduced mononuclear leukocyte immunoreactive levels of G(s)α and G(i)α proteins, similar to previous observations in patients with nonseasonal major depression. The reduced G protein levels were normalized with 2 weeks of light therapy. The same patients while in remission during the summer had G protein levels that were similar to those of healthy subjects. Conclusions: G protein-immunoreactive measures in patients with SAD are suggested as a state marker for winter depression, which is normalized by light treatment and during the summer. We speculate that light may exert its effects via normalization of transducing (G1 protein) levels, which are thought to be reduced in winter depression.

AB - Background: Information-transducing heterotrimeric G proteins have been implicated previously in the mechanism of action of mood stabilizers and in the pathophysiology of mood disorders, Mononuclear leukocytes of patients with unipolar and bipolar depression have been characterized by reduced measures of the stimulatory and inhibitory G proteins. In this study, patients with seasonal affective disorder (SAD) were measured for mononuclear leukocyte G protein levels while depressed during the winter, following light therapy, and in remission during the summer. Methods: Twenty-six patients with SAD and 28 healthy subjects were assessed in the study. The immunoreactivities of G5α, G1α, and Gβ subunit proteins were determined by Western blot analysis of mononuclear leukocyte membranes with selective polyclonal antibodies for the various G subunit proteins, followed by densitometric quantitation using an image analysis system. Results: Untreated patients with SAD and winter, atypical-type depression showed significantly reduced mononuclear leukocyte immunoreactive levels of G(s)α and G(i)α proteins, similar to previous observations in patients with nonseasonal major depression. The reduced G protein levels were normalized with 2 weeks of light therapy. The same patients while in remission during the summer had G protein levels that were similar to those of healthy subjects. Conclusions: G protein-immunoreactive measures in patients with SAD are suggested as a state marker for winter depression, which is normalized by light treatment and during the summer. We speculate that light may exert its effects via normalization of transducing (G1 protein) levels, which are thought to be reduced in winter depression.

UR - http://www.scopus.com/inward/record.url?scp=0033042796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033042796&partnerID=8YFLogxK

U2 - 10.1001/archpsyc.56.2.178

DO - 10.1001/archpsyc.56.2.178

M3 - Article

VL - 56

SP - 178

EP - 183

JO - JAMA Psychiatry

JF - JAMA Psychiatry

SN - 2168-622X

IS - 2

ER -