Salicylate inhibited the response of human peripheral lymphocytes to phytohemagglutinim (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and X-irradiated allogeneic lymphocytes. In addition, salicylate suppressed the generation of cytotoxic lymphocytes (CL) in vitro. The effect was dose dependent with over 90% inhibition at 48 mg%. Once CL were generated, however, the drug had little effect on their ability to lyse target cells. Sodium salicylate also suppressed the proliferative response of parental mouse thymocytes adoptively transferred into lethally irradiated F1 hybrid recipients as assessed by splenic uptake of [125I] iododeoxyuridine. High-dose, chronic treatment of both thymocyte donors and recipients was necessary to effect a 20-60% suppression. Similar chronic treatment of mice depressed the number of splenic plaque-forming cells by 48-72% when assayed 4 days after primary challenge with sheep erythrocytes. On the contrary, we could find no effect of phenobarbital on the response to PHA, mixed lymphocyte culture, or on the generation of CL.
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine