Abstract
To determine the effect of steroid occupancy of the glucocorticoid receptor on the measurement of total receptor concentration using Scatchard plot of [3H]dexamethasone (Dex) binding to circulating leukocytes, leukocytes were incubated with 10-7 M cortisol or 10-9 M Dex prior to [3H]Dex binding study. Total binding capacity calculated from Scatchard plot analysis of [3H]Dex binding was not significantly reduced after pre-incubation with cortisol. However, total binding capacity was significantly lower after pre-incubation with Dex. These data suggest that total glucocorticoid receptor concentration can be measured from a 3 hr incubation of leukocytes obtained from untreated patients.
Original language | English (US) |
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Pages (from-to) | 376-380 |
Number of pages | 5 |
Journal | Acta Endocrinologica |
Volume | 94 |
Issue number | 3 |
State | Published - 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Endocrinology