Effects of receptor occupancy on [3H]dexamethasone binding to circulating leukocytes

T. Murakami, D. D. Brandon, Donald (Lynn) Loriaux, M. B. Lipsett

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To determine the effect of steroid occupancy of the glucocorticoid receptor on the measurement of total receptor concentration using Scatchard plot of [3H]dexamethasone (Dex) binding to circulating leukocytes, leukocytes were incubated with 10-7 M cortisol or 10-9 M Dex prior to [3H]Dex binding study. Total binding capacity calculated from Scatchard plot analysis of [3H]Dex binding was not significantly reduced after pre-incubation with cortisol. However, total binding capacity was significantly lower after pre-incubation with Dex. These data suggest that total glucocorticoid receptor concentration can be measured from a 3 hr incubation of leukocytes obtained from untreated patients.

Original languageEnglish (US)
Pages (from-to)376-380
Number of pages5
JournalActa Endocrinologica
Volume94
Issue number3
StatePublished - 1980
Externally publishedYes

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Dexamethasone
Leukocytes
Glucocorticoid Receptors
Hydrocortisone
Steroids

ASJC Scopus subject areas

  • Endocrinology

Cite this

Effects of receptor occupancy on [3H]dexamethasone binding to circulating leukocytes. / Murakami, T.; Brandon, D. D.; Loriaux, Donald (Lynn); Lipsett, M. B.

In: Acta Endocrinologica, Vol. 94, No. 3, 1980, p. 376-380.

Research output: Contribution to journalArticle

Murakami, T, Brandon, DD, Loriaux, DL & Lipsett, MB 1980, 'Effects of receptor occupancy on [3H]dexamethasone binding to circulating leukocytes', Acta Endocrinologica, vol. 94, no. 3, pp. 376-380.
Murakami, T. ; Brandon, D. D. ; Loriaux, Donald (Lynn) ; Lipsett, M. B. / Effects of receptor occupancy on [3H]dexamethasone binding to circulating leukocytes. In: Acta Endocrinologica. 1980 ; Vol. 94, No. 3. pp. 376-380.
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