Effects of plasmid propagation of a sporulation promoter on promoter utilization and sporulation in Bacillus subtilis

Peter Zuber, J. M. Healy, R. Losick

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Transcription of the sporulation gene spoVG of Bacillus subtilis is induced at the onset of spore formation and depends on the products of the regulatory genes spo0A, spo0B, and spo0H. We describe two effects of propagating the promoter region of spoVG on a multicopy plasmid replicon in B. subtilis cells. One effect is that transcription from the plasmid-borne spoVG promoter is altered with respect to the time of its induction and the dependence on spo0 gene products. An example of this effect is that plasmid propagation was observed to relieve substantially the inhibitory effect of a mutation in spo0H, the spo0 gene upon which spoVG promoter activity is most strongly dependent. We present results which suggest that propagation on a plasmid replicon causes an alteration in the conformation of spoVG promoter DNA which somehow compensates for the defective spo0H gene product. Plasmid propagation did not, however, entirely eliminate the requirement for the spo0H gene product; little or no spoVG-directed RNA synthesis was observed in cells bearing a putative spo0H deletion mutation, a finding which indicates that Spo0H protein plays an indispensable role in spoVG promoter utilization. Another effect of propagating the promoter region of spoVG on a multicopy plasmid is to inhibit sporulation. S1 nuclease mapping experiments suggest that amplification of spoVG on a multicopy plasmid causes the titration of a transcription factor or minor form of RNA polymerase holoenzyme required for utilization of one of the two overlapping promoters which comprise the spoVG transcription initiation region.

Original languageEnglish (US)
Pages (from-to)461-469
Number of pages9
JournalJournal of Bacteriology
Volume169
Issue number2
StatePublished - 1987
Externally publishedYes

Fingerprint

Bacillus subtilis
Plasmids
Replicon
Genes
Genetic Promoter Regions
Holoenzymes
Sequence Deletion
DNA-Directed RNA Polymerases
Regulator Genes
Spores
Transcription Factors
RNA
Mutation
DNA
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Effects of plasmid propagation of a sporulation promoter on promoter utilization and sporulation in Bacillus subtilis. / Zuber, Peter; Healy, J. M.; Losick, R.

In: Journal of Bacteriology, Vol. 169, No. 2, 1987, p. 461-469.

Research output: Contribution to journalArticle

@article{43e321413ff84fcd94720fb0718c7945,
title = "Effects of plasmid propagation of a sporulation promoter on promoter utilization and sporulation in Bacillus subtilis",
abstract = "Transcription of the sporulation gene spoVG of Bacillus subtilis is induced at the onset of spore formation and depends on the products of the regulatory genes spo0A, spo0B, and spo0H. We describe two effects of propagating the promoter region of spoVG on a multicopy plasmid replicon in B. subtilis cells. One effect is that transcription from the plasmid-borne spoVG promoter is altered with respect to the time of its induction and the dependence on spo0 gene products. An example of this effect is that plasmid propagation was observed to relieve substantially the inhibitory effect of a mutation in spo0H, the spo0 gene upon which spoVG promoter activity is most strongly dependent. We present results which suggest that propagation on a plasmid replicon causes an alteration in the conformation of spoVG promoter DNA which somehow compensates for the defective spo0H gene product. Plasmid propagation did not, however, entirely eliminate the requirement for the spo0H gene product; little or no spoVG-directed RNA synthesis was observed in cells bearing a putative spo0H deletion mutation, a finding which indicates that Spo0H protein plays an indispensable role in spoVG promoter utilization. Another effect of propagating the promoter region of spoVG on a multicopy plasmid is to inhibit sporulation. S1 nuclease mapping experiments suggest that amplification of spoVG on a multicopy plasmid causes the titration of a transcription factor or minor form of RNA polymerase holoenzyme required for utilization of one of the two overlapping promoters which comprise the spoVG transcription initiation region.",
author = "Peter Zuber and Healy, {J. M.} and R. Losick",
year = "1987",
language = "English (US)",
volume = "169",
pages = "461--469",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Effects of plasmid propagation of a sporulation promoter on promoter utilization and sporulation in Bacillus subtilis

AU - Zuber, Peter

AU - Healy, J. M.

AU - Losick, R.

PY - 1987

Y1 - 1987

N2 - Transcription of the sporulation gene spoVG of Bacillus subtilis is induced at the onset of spore formation and depends on the products of the regulatory genes spo0A, spo0B, and spo0H. We describe two effects of propagating the promoter region of spoVG on a multicopy plasmid replicon in B. subtilis cells. One effect is that transcription from the plasmid-borne spoVG promoter is altered with respect to the time of its induction and the dependence on spo0 gene products. An example of this effect is that plasmid propagation was observed to relieve substantially the inhibitory effect of a mutation in spo0H, the spo0 gene upon which spoVG promoter activity is most strongly dependent. We present results which suggest that propagation on a plasmid replicon causes an alteration in the conformation of spoVG promoter DNA which somehow compensates for the defective spo0H gene product. Plasmid propagation did not, however, entirely eliminate the requirement for the spo0H gene product; little or no spoVG-directed RNA synthesis was observed in cells bearing a putative spo0H deletion mutation, a finding which indicates that Spo0H protein plays an indispensable role in spoVG promoter utilization. Another effect of propagating the promoter region of spoVG on a multicopy plasmid is to inhibit sporulation. S1 nuclease mapping experiments suggest that amplification of spoVG on a multicopy plasmid causes the titration of a transcription factor or minor form of RNA polymerase holoenzyme required for utilization of one of the two overlapping promoters which comprise the spoVG transcription initiation region.

AB - Transcription of the sporulation gene spoVG of Bacillus subtilis is induced at the onset of spore formation and depends on the products of the regulatory genes spo0A, spo0B, and spo0H. We describe two effects of propagating the promoter region of spoVG on a multicopy plasmid replicon in B. subtilis cells. One effect is that transcription from the plasmid-borne spoVG promoter is altered with respect to the time of its induction and the dependence on spo0 gene products. An example of this effect is that plasmid propagation was observed to relieve substantially the inhibitory effect of a mutation in spo0H, the spo0 gene upon which spoVG promoter activity is most strongly dependent. We present results which suggest that propagation on a plasmid replicon causes an alteration in the conformation of spoVG promoter DNA which somehow compensates for the defective spo0H gene product. Plasmid propagation did not, however, entirely eliminate the requirement for the spo0H gene product; little or no spoVG-directed RNA synthesis was observed in cells bearing a putative spo0H deletion mutation, a finding which indicates that Spo0H protein plays an indispensable role in spoVG promoter utilization. Another effect of propagating the promoter region of spoVG on a multicopy plasmid is to inhibit sporulation. S1 nuclease mapping experiments suggest that amplification of spoVG on a multicopy plasmid causes the titration of a transcription factor or minor form of RNA polymerase holoenzyme required for utilization of one of the two overlapping promoters which comprise the spoVG transcription initiation region.

UR - http://www.scopus.com/inward/record.url?scp=0023149314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023149314&partnerID=8YFLogxK

M3 - Article

C2 - 3027029

AN - SCOPUS:0023149314

VL - 169

SP - 461

EP - 469

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 2

ER -