Effects of nucleocapsid mutations on human immunodeficiency virus assembly and RNA encapsidation

Yaqiang Zhang, Eric Barklis

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The human immunodeficiency virus (HIV) Pr55(Gag) precursor proteins direct virus particle assembly. While Gag-Gag protein interactions which affect HIV assembly occur in the capsid (CA) domain of Pr55(Gag), the nucleocapsid (NC) domain, which functions in viral RNA encapsidation, also appears to participate in virus assembly. In order to dissect the roles of the NC domain and the p6 domain, the C-terminal Gag protein domain, we examined the effects of NC and p6 mutations on virus assembly and RNA encapsidation. In our experimental system, the p6 domain did not appear to affect virus release efficiency but p6 deletions and truncations reduced the specificity of genomic HIV-1 RNA encapsidation. Mutations in the nucleocapsid region reduced particle release, especially when the p2 interdomain peptide or the amino-terminal portion of the NC region was mutated, and NC mutations also reduced both the specificity and the efficiency of HIV-1 RNA encapsidation. These results implicated a linkage between RNA encapsidation and virus particle assembly or release. However, we found that the mutant ApoMTRB, in which the nucleocapsid and p6 domains of HIV-1 Pr55(Gag) were replaced with the Bacillus sabtilis MtrB protein domain, released particles efficiently but packaged no detectable RNA. These results suggest that, for the purposes of virus-like particle assembly and release, NC can be replaced by a protein that does not appear to encapsidate RNA.

Original languageEnglish (US)
Pages (from-to)6765-6776
Number of pages12
JournalJournal of Virology
Volume71
Issue number9
StatePublished - 1997

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Virus Assembly
Nucleocapsid
nucleocapsid
Human immunodeficiency virus
HIV
RNA
mutation
Mutation
gag Gene Products
Human immunodeficiency virus 1
Virion
Virus Release
HIV-1
virion
proteins
virus-like particles
capsid
virus assembly
Capsid
RNA Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Effects of nucleocapsid mutations on human immunodeficiency virus assembly and RNA encapsidation. / Zhang, Yaqiang; Barklis, Eric.

In: Journal of Virology, Vol. 71, No. 9, 1997, p. 6765-6776.

Research output: Contribution to journalArticle

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