Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium

A. Stanek, Ansgar Brambrink, F. Latorre, B. Bender, P. P. Kleemann

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Respiratory infection is a major cause of morbidity after general anaesthesia. Impairment of respiratory ciliary beat frequency (CBF) by different stress factors causes a decrease in mucus transport rate (MTR). We have tested the effect of different concentrations of oxygen on CBF of human respiratory epithelium in a prospective, randomized, in vitro study. Samples of superficial mucosa of the inferior nasal turbinates of 20 non-smoking healthy volunteers were harvested and exposed to three different oxygen environments (group I = 21% oxygen, group II = 60% oxygen and group III = 95% oxygen) for 2 h. In 50% of the samples, exposure time was prolonged. At 30, 60, 90, 120 and 240 min, light microscopic images of cilia activity were videotaped and CBF was later assessed in slow motion. Compared with baseline, group showed no difference in CBF throughout the study. CBF was increased in group II from mean 9.7 (SD 0.4) to 11.2 (0.4) Hz (16%, P <0.001) and in group III from 9.5 (0.6) to 12.1 (0.5) Hz (28%, P <0.001) at 120 min. After 240 min of exposure to 95% oxygen, the CBF trend in group III was reduced to 11.8 (0.6) Hz but still remained above baseline. We conclude that oxygen appeared to have a dose- and time-dependent accelerating effect on CBF. Prolonged exposure to high oxygen concentrations reversed this trend. Direct oxygen toxicity ('oxygen stress') is a possible explanation for this effect. These changes may result in impaired MTR.

Original languageEnglish (US)
Pages (from-to)660-664
Number of pages5
JournalBritish Journal of Anaesthesia
Volume80
Issue number5
StatePublished - May 1998
Externally publishedYes

Fingerprint

Respiratory Mucosa
Oxygen
Mucus
Turbinates
Nasal Mucosa
Cilia
Respiratory Tract Infections
General Anesthesia
Healthy Volunteers
Morbidity
Light

Keywords

  • Lung, mucus
  • Lung, trachea
  • Oxygen, toxicity

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Stanek, A., Brambrink, A., Latorre, F., Bender, B., & Kleemann, P. P. (1998). Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium. British Journal of Anaesthesia, 80(5), 660-664.

Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium. / Stanek, A.; Brambrink, Ansgar; Latorre, F.; Bender, B.; Kleemann, P. P.

In: British Journal of Anaesthesia, Vol. 80, No. 5, 05.1998, p. 660-664.

Research output: Contribution to journalArticle

Stanek, A, Brambrink, A, Latorre, F, Bender, B & Kleemann, PP 1998, 'Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium', British Journal of Anaesthesia, vol. 80, no. 5, pp. 660-664.
Stanek A, Brambrink A, Latorre F, Bender B, Kleemann PP. Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium. British Journal of Anaesthesia. 1998 May;80(5):660-664.
Stanek, A. ; Brambrink, Ansgar ; Latorre, F. ; Bender, B. ; Kleemann, P. P. / Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium. In: British Journal of Anaesthesia. 1998 ; Vol. 80, No. 5. pp. 660-664.
@article{5ee54064d105493c91deca578007a198,
title = "Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium",
abstract = "Respiratory infection is a major cause of morbidity after general anaesthesia. Impairment of respiratory ciliary beat frequency (CBF) by different stress factors causes a decrease in mucus transport rate (MTR). We have tested the effect of different concentrations of oxygen on CBF of human respiratory epithelium in a prospective, randomized, in vitro study. Samples of superficial mucosa of the inferior nasal turbinates of 20 non-smoking healthy volunteers were harvested and exposed to three different oxygen environments (group I = 21{\%} oxygen, group II = 60{\%} oxygen and group III = 95{\%} oxygen) for 2 h. In 50{\%} of the samples, exposure time was prolonged. At 30, 60, 90, 120 and 240 min, light microscopic images of cilia activity were videotaped and CBF was later assessed in slow motion. Compared with baseline, group showed no difference in CBF throughout the study. CBF was increased in group II from mean 9.7 (SD 0.4) to 11.2 (0.4) Hz (16{\%}, P <0.001) and in group III from 9.5 (0.6) to 12.1 (0.5) Hz (28{\%}, P <0.001) at 120 min. After 240 min of exposure to 95{\%} oxygen, the CBF trend in group III was reduced to 11.8 (0.6) Hz but still remained above baseline. We conclude that oxygen appeared to have a dose- and time-dependent accelerating effect on CBF. Prolonged exposure to high oxygen concentrations reversed this trend. Direct oxygen toxicity ('oxygen stress') is a possible explanation for this effect. These changes may result in impaired MTR.",
keywords = "Lung, mucus, Lung, trachea, Oxygen, toxicity",
author = "A. Stanek and Ansgar Brambrink and F. Latorre and B. Bender and Kleemann, {P. P.}",
year = "1998",
month = "5",
language = "English (US)",
volume = "80",
pages = "660--664",
journal = "British Journal of Anaesthesia",
issn = "0007-0912",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium

AU - Stanek, A.

AU - Brambrink, Ansgar

AU - Latorre, F.

AU - Bender, B.

AU - Kleemann, P. P.

PY - 1998/5

Y1 - 1998/5

N2 - Respiratory infection is a major cause of morbidity after general anaesthesia. Impairment of respiratory ciliary beat frequency (CBF) by different stress factors causes a decrease in mucus transport rate (MTR). We have tested the effect of different concentrations of oxygen on CBF of human respiratory epithelium in a prospective, randomized, in vitro study. Samples of superficial mucosa of the inferior nasal turbinates of 20 non-smoking healthy volunteers were harvested and exposed to three different oxygen environments (group I = 21% oxygen, group II = 60% oxygen and group III = 95% oxygen) for 2 h. In 50% of the samples, exposure time was prolonged. At 30, 60, 90, 120 and 240 min, light microscopic images of cilia activity were videotaped and CBF was later assessed in slow motion. Compared with baseline, group showed no difference in CBF throughout the study. CBF was increased in group II from mean 9.7 (SD 0.4) to 11.2 (0.4) Hz (16%, P <0.001) and in group III from 9.5 (0.6) to 12.1 (0.5) Hz (28%, P <0.001) at 120 min. After 240 min of exposure to 95% oxygen, the CBF trend in group III was reduced to 11.8 (0.6) Hz but still remained above baseline. We conclude that oxygen appeared to have a dose- and time-dependent accelerating effect on CBF. Prolonged exposure to high oxygen concentrations reversed this trend. Direct oxygen toxicity ('oxygen stress') is a possible explanation for this effect. These changes may result in impaired MTR.

AB - Respiratory infection is a major cause of morbidity after general anaesthesia. Impairment of respiratory ciliary beat frequency (CBF) by different stress factors causes a decrease in mucus transport rate (MTR). We have tested the effect of different concentrations of oxygen on CBF of human respiratory epithelium in a prospective, randomized, in vitro study. Samples of superficial mucosa of the inferior nasal turbinates of 20 non-smoking healthy volunteers were harvested and exposed to three different oxygen environments (group I = 21% oxygen, group II = 60% oxygen and group III = 95% oxygen) for 2 h. In 50% of the samples, exposure time was prolonged. At 30, 60, 90, 120 and 240 min, light microscopic images of cilia activity were videotaped and CBF was later assessed in slow motion. Compared with baseline, group showed no difference in CBF throughout the study. CBF was increased in group II from mean 9.7 (SD 0.4) to 11.2 (0.4) Hz (16%, P <0.001) and in group III from 9.5 (0.6) to 12.1 (0.5) Hz (28%, P <0.001) at 120 min. After 240 min of exposure to 95% oxygen, the CBF trend in group III was reduced to 11.8 (0.6) Hz but still remained above baseline. We conclude that oxygen appeared to have a dose- and time-dependent accelerating effect on CBF. Prolonged exposure to high oxygen concentrations reversed this trend. Direct oxygen toxicity ('oxygen stress') is a possible explanation for this effect. These changes may result in impaired MTR.

KW - Lung, mucus

KW - Lung, trachea

KW - Oxygen, toxicity

UR - http://www.scopus.com/inward/record.url?scp=0031976399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031976399&partnerID=8YFLogxK

M3 - Article

VL - 80

SP - 660

EP - 664

JO - British Journal of Anaesthesia

JF - British Journal of Anaesthesia

SN - 0007-0912

IS - 5

ER -