TY - JOUR
T1 - Effects of nicotine on brain 1-phosphatidylinositol-4-phosphate and 1-phosphatidylinositol-3,4-bisphosphate synthesis and metabolism-possible relationship to nicotine-induced behaviors
AU - Hitzemann, Robert J.
AU - Natsuki, Reiko
AU - Loh, Horace H.
N1 - Funding Information:
Acknowledgements-Thea uthorsa cknowledgteh etech-nical assistancoef BarbaraH itzemanna nd thee ditorial and typing assistanceo f BarbaraH alperin and Kaye Welch. This study was supported in part by USPHS
PY - 1978
Y1 - 1978
N2 - The effects of nicotine and other cholinergic drugs on the initial incorporation of 32Pi, and [3H]glycerol into 1-phosphatidylinositol-4-phosphate(DPI)and 1-phosphatidylinsitol-3,4-bisphosphate (TPI) in the rat brain microsomal fraction were studied. Nicotine and eserine significantly decreased and mecamylamine increased [32P]- and [3H]TPI levels. Atropine had no effect on labeled TPI levels. Dose-effect studies for nicotine revealed that as little as 0.1 mg/kg of nicotine, i.p., significantly decreased the accumulation of labeled TPI. It was concluded that pharmacologically relevant and nonconvulsive doses of nicotine can markedly affect brain polyphosphoinositide synthesis and/or metabolism; the nature of the effects is consistent with theories suggesting that the conversion of TPI to DPI will release membrane lipid bound Ca2+ and, thus, increase membrane excitability.
AB - The effects of nicotine and other cholinergic drugs on the initial incorporation of 32Pi, and [3H]glycerol into 1-phosphatidylinositol-4-phosphate(DPI)and 1-phosphatidylinsitol-3,4-bisphosphate (TPI) in the rat brain microsomal fraction were studied. Nicotine and eserine significantly decreased and mecamylamine increased [32P]- and [3H]TPI levels. Atropine had no effect on labeled TPI levels. Dose-effect studies for nicotine revealed that as little as 0.1 mg/kg of nicotine, i.p., significantly decreased the accumulation of labeled TPI. It was concluded that pharmacologically relevant and nonconvulsive doses of nicotine can markedly affect brain polyphosphoinositide synthesis and/or metabolism; the nature of the effects is consistent with theories suggesting that the conversion of TPI to DPI will release membrane lipid bound Ca2+ and, thus, increase membrane excitability.
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U2 - 10.1016/0006-2952(78)90319-2
DO - 10.1016/0006-2952(78)90319-2
M3 - Article
C2 - 215157
AN - SCOPUS:0018186221
SN - 0006-2952
VL - 27
SP - 2519
EP - 2523
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 21
ER -