Effects of naloxone on d-amphetamine- and apomorphine-induced behavior

Robert Hitzemann, J. Curell, D. Hom, H. Loh

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The effects of acute naloxone administration on d-amphetamine- and apomorphine-induced behavior were studied. Naloxone, in doses of 0.3-10 mg/kg (s.c.), antagonized the increase in ambulation and rearing induced by 1 mg/kg of d-amphetamine. When the dose of d-amphetamine was increased to 3 mg/kg, naloxone (3 mg/kg) antagonized only the increase in rearing activity. No dose (0.3-10 mg/kg, s.c.) of naloxone significantly affected d-amphetamine- or apomorphine-induced stereotyped activity. Naloxone (3 mg/kg) significantly augmented the apomorphine (1 mg/kg. s.c.)-induced increase in ambulation but attenuated the apomorphine (0.3 mg/kg)-induced increase in rearing activity. Naloxone (3 mg/kg) or apomorphine (0.03 mg/kg) significantly decreased the ambulation and rearing induced by a novel environment. In combination and in these doses, naloxone and apomorphine produced an additive effect on these behaviors. The neurochemical mechanisms by which naloxone affects d-amphetamine- and apomorphine-induced behavior were investigated. Naloxone (10-6M) had no significant effect on [3H]spiroperidol binding in either the caudate nucleus or nucleus accumbens except for a modest inhibition (24%) of both the Km and Bmax in the accumbens microsomal fraction. Similarly, naloxone (10-6 M) had no significant effect on [3H]dopamine(DA) uptake into either brain region nor did naloxone alter the d-amphetamine-inhibition of uptake. Using perfused tissue slices, naloxone (10-6-10-5 M) significantly attenuated the increase in [3H]DA release induced by d-amphetamine (10-5 M) in both brain regions. Naloxone (1 mg/kg) had no significant effect on DA or dihydroxyphenylacetic acid (DOPAC) levels or on the DA/DOPAC ratio in the caudate nucleus or nucleus accumbens. However, naloxone did reverse the marked increases in the DA-DOPAC ratio induced by d-amphet-amine (1 mg/kg) in both brain regions.

Original languageEnglish (US)
Pages (from-to)1005-1011
Number of pages7
JournalNeuropharmacology
Volume21
Issue number10
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Dextroamphetamine
Apomorphine
Naloxone
Dopamine
Walking
Caudate Nucleus
Nucleus Accumbens
Acids
Brain
Spiperone
Amines

Keywords

  • apomorphine
  • behavior
  • D-amphetamine
  • dopamine
  • naloxone

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Effects of naloxone on d-amphetamine- and apomorphine-induced behavior. / Hitzemann, Robert; Curell, J.; Hom, D.; Loh, H.

In: Neuropharmacology, Vol. 21, No. 10, 1982, p. 1005-1011.

Research output: Contribution to journalArticle

Hitzemann, Robert ; Curell, J. ; Hom, D. ; Loh, H. / Effects of naloxone on d-amphetamine- and apomorphine-induced behavior. In: Neuropharmacology. 1982 ; Vol. 21, No. 10. pp. 1005-1011.
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