Effects of methotrexate on transfected DNA stability in mammalian cells

Maria G. Pallavicini, Patricia S. Deteresa, Caridad Rosette, Joe Gray, Florian M. Wurm

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The chromosomal locations, amounts, and level of expression of transfected, amplified c-myc and dihydrofolate reductase sequences were measured in cells cultured in the presence and absence of methotrexate. These studies show that the location and amount of transfected sequences, as well as the level of expression, were more variable when the cells were cultured in methotrexate.

Original languageEnglish (US)
Pages (from-to)401-404
Number of pages4
JournalMolecular and Cellular Biology
Volume10
Issue number1
StatePublished - Jan 1990
Externally publishedYes

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Methotrexate
Cultured Cells
Tetrahydrofolate Dehydrogenase
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Pallavicini, M. G., Deteresa, P. S., Rosette, C., Gray, J., & Wurm, F. M. (1990). Effects of methotrexate on transfected DNA stability in mammalian cells. Molecular and Cellular Biology, 10(1), 401-404.

Effects of methotrexate on transfected DNA stability in mammalian cells. / Pallavicini, Maria G.; Deteresa, Patricia S.; Rosette, Caridad; Gray, Joe; Wurm, Florian M.

In: Molecular and Cellular Biology, Vol. 10, No. 1, 01.1990, p. 401-404.

Research output: Contribution to journalArticle

Pallavicini, MG, Deteresa, PS, Rosette, C, Gray, J & Wurm, FM 1990, 'Effects of methotrexate on transfected DNA stability in mammalian cells', Molecular and Cellular Biology, vol. 10, no. 1, pp. 401-404.
Pallavicini MG, Deteresa PS, Rosette C, Gray J, Wurm FM. Effects of methotrexate on transfected DNA stability in mammalian cells. Molecular and Cellular Biology. 1990 Jan;10(1):401-404.
Pallavicini, Maria G. ; Deteresa, Patricia S. ; Rosette, Caridad ; Gray, Joe ; Wurm, Florian M. / Effects of methotrexate on transfected DNA stability in mammalian cells. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 1. pp. 401-404.
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