Abstract
Pigs experience significant conceptus loss near mid-gestation, correlating with increasing glandular epithelial (GE) development and secretory activity. Secreted phosphoprotein 1 (SPP1, osteopontin) increases in GE between days 30 and 40 of pregnancy and is expressed in the GE of day 90 pseudopregnant pigs, suggesting that progesterone (P4) from corpora lutea is responsible for induction of SPP1 in GE. In this study, pigs were ovariectomized and treated daily with P4 to assess effects of 40 days of P4 exposure on SPP1, P4 receptor (PGR), uteroferrin (ACP5), and fibroblast growth factor 7 (FGF7) expression in porcine endometria. PGR mRNA decreased in pigs injected with P4 compared with pigs injected with corn oil (CO), and PGRs were downregulated in the luminal epithelium (LE) and GE. ACP5 mRNA increased in pigs injected with P4 compared with pigs injected with CO, and ACP5 was induced in the GE of P4-treated pigs. FGF7 mRNA increased in pigs injected with P4 compared with pigs injected with CO, and FGF7 was induced in the LE and GE of P4-treated pigs. SPP1 mRNAwas not different between pigs injected with P4 compared with pigs injected with CO, and SPP1 was not present in the GE of P 4-treated pigs. Therefore, long-term P4, in the absence of ovarian and/or conceptus factors, does not induce SPP1 expression in GE. We hypothesize that a servomechanism involving sequential effects of multiple hormones and cytokines, similar to those for sheep and humans, is required for GE differentiation and function, including the synthesis and secretion of SPP1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 595-604 |
| Number of pages | 10 |
| Journal | Reproduction |
| Volume | 140 |
| Issue number | 4 |
| DOIs | |
| State | Published - Oct 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine
- Embryology
- Endocrinology
- Cell Biology
Cite this
Effects of long-term progesterone exposure on porcine uterine gene expression : Progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium. / Bailey, Daniel W.; Dunlap, Kathrin A.; Erikson, David; Patel, Atish K.; Bazer, Fuller W.; Burghardt, Robert C.; Johnson, Greg A.
In: Reproduction, Vol. 140, No. 4, 10.2010, p. 595-604.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of long-term progesterone exposure on porcine uterine gene expression
T2 - Progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium
AU - Bailey, Daniel W.
AU - Dunlap, Kathrin A.
AU - Erikson, David
AU - Patel, Atish K.
AU - Bazer, Fuller W.
AU - Burghardt, Robert C.
AU - Johnson, Greg A.
PY - 2010/10
Y1 - 2010/10
N2 - Pigs experience significant conceptus loss near mid-gestation, correlating with increasing glandular epithelial (GE) development and secretory activity. Secreted phosphoprotein 1 (SPP1, osteopontin) increases in GE between days 30 and 40 of pregnancy and is expressed in the GE of day 90 pseudopregnant pigs, suggesting that progesterone (P4) from corpora lutea is responsible for induction of SPP1 in GE. In this study, pigs were ovariectomized and treated daily with P4 to assess effects of 40 days of P4 exposure on SPP1, P4 receptor (PGR), uteroferrin (ACP5), and fibroblast growth factor 7 (FGF7) expression in porcine endometria. PGR mRNA decreased in pigs injected with P4 compared with pigs injected with corn oil (CO), and PGRs were downregulated in the luminal epithelium (LE) and GE. ACP5 mRNA increased in pigs injected with P4 compared with pigs injected with CO, and ACP5 was induced in the GE of P4-treated pigs. FGF7 mRNA increased in pigs injected with P4 compared with pigs injected with CO, and FGF7 was induced in the LE and GE of P4-treated pigs. SPP1 mRNAwas not different between pigs injected with P4 compared with pigs injected with CO, and SPP1 was not present in the GE of P 4-treated pigs. Therefore, long-term P4, in the absence of ovarian and/or conceptus factors, does not induce SPP1 expression in GE. We hypothesize that a servomechanism involving sequential effects of multiple hormones and cytokines, similar to those for sheep and humans, is required for GE differentiation and function, including the synthesis and secretion of SPP1.
AB - Pigs experience significant conceptus loss near mid-gestation, correlating with increasing glandular epithelial (GE) development and secretory activity. Secreted phosphoprotein 1 (SPP1, osteopontin) increases in GE between days 30 and 40 of pregnancy and is expressed in the GE of day 90 pseudopregnant pigs, suggesting that progesterone (P4) from corpora lutea is responsible for induction of SPP1 in GE. In this study, pigs were ovariectomized and treated daily with P4 to assess effects of 40 days of P4 exposure on SPP1, P4 receptor (PGR), uteroferrin (ACP5), and fibroblast growth factor 7 (FGF7) expression in porcine endometria. PGR mRNA decreased in pigs injected with P4 compared with pigs injected with corn oil (CO), and PGRs were downregulated in the luminal epithelium (LE) and GE. ACP5 mRNA increased in pigs injected with P4 compared with pigs injected with CO, and ACP5 was induced in the GE of P4-treated pigs. FGF7 mRNA increased in pigs injected with P4 compared with pigs injected with CO, and FGF7 was induced in the LE and GE of P4-treated pigs. SPP1 mRNAwas not different between pigs injected with P4 compared with pigs injected with CO, and SPP1 was not present in the GE of P 4-treated pigs. Therefore, long-term P4, in the absence of ovarian and/or conceptus factors, does not induce SPP1 expression in GE. We hypothesize that a servomechanism involving sequential effects of multiple hormones and cytokines, similar to those for sheep and humans, is required for GE differentiation and function, including the synthesis and secretion of SPP1.
UR - http://www.scopus.com/inward/record.url?scp=77957991648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957991648&partnerID=8YFLogxK
U2 - 10.1530/REP-10-0169
DO - 10.1530/REP-10-0169
M3 - Article
C2 - 20705772
AN - SCOPUS:77957991648
VL - 140
SP - 595
EP - 604
JO - Reproduction
JF - Reproduction
SN - 1470-1626
IS - 4
ER -