Effects of lipoic acid on primary murine microglial cells

Priya Chaudhary, Gail Marracci, Edvinas Pocius, Danielle Galipeau, Brooke Morris, Dennis Bourdette

Research output: Contribution to journalArticle

Abstract

The anti-oxidant lipoic acid (LA) is beneficial in murine models of multiple sclerosis (MS) and has recently been shown to slow brain atrophy in secondary progressive MS. The mechanism of these effects by LA is incompletely understood but may involve effects on microglia. The objective of this study is to understand how LA affects microglial cells. We cultured primary microglial cells from C57BL/6 adult mice brains and stimulated the cells with lipopolysaccharide (LPS) and interferon gamma (IFN-γ) in the presence or absence of LA. We demonstrate the inhibition of phagocytosis, rearrangement of actin, and formation of membrane blebs in stimulated microglia in the presence of LA. These experiments suggest that LA causes changes in microglial actin, which may lead to alterations in phagocytosis, mobility, and migration.

Original languageEnglish (US)
Article number576972
JournalJournal of Neuroimmunology
Volume334
DOIs
StatePublished - Sep 15 2019

Fingerprint

Thioctic Acid
Microglia
Phagocytosis
Actins
Chronic Progressive Multiple Sclerosis
Brain
Blister
Oxidants
Interferon-gamma
Multiple Sclerosis
Atrophy
Lipopolysaccharides
Membranes

Keywords

  • Actin
  • Blebs
  • Cytoskeleton
  • Microglia
  • Phagocytosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Effects of lipoic acid on primary murine microglial cells. / Chaudhary, Priya; Marracci, Gail; Pocius, Edvinas; Galipeau, Danielle; Morris, Brooke; Bourdette, Dennis.

In: Journal of Neuroimmunology, Vol. 334, 576972, 15.09.2019.

Research output: Contribution to journalArticle

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AU - Bourdette, Dennis

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