Effects of intravenous pentafraction on lung and soft tissue liquid exchange in hypoproteinemic sheep

R. L. Conhaim, D. J. Rosenfeld, Martin Schreiber, D. M. Baaske, B. A. Harms

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Effects of infusing pentafraction (Pen), a synthetic hydroxyethyl starch plasma volume expander, on lung and soft tissue lymph flux were compared in nonanesthetized sheep that were protein depleted by batch plasmapheresis. Pen (5%) was infused to raise pulmonary arterial wedge pressure by 5 mmHg for 2 h (1.8 ± 0.3 l). Pen raised plasma osmotic pressure from plasmapheresis baseline (10.7 ± 2.2 mmHg; preplasmapheresis baseline, 19.6 ± 0.6 mmHg) to 18.6 ± 2.4 mmHg. After Pen, lung lymph flows peaked at 3.9 ± 2.0 times a preplasmapheresis baseline value of 1.0 (plasmapheresis baseline, 2.7 ± 0.7), but soft tissue lymph flows rose insignificantly. Plasma Pen concentrations were 2.3 ± 1.0% postinfusion and 1.6 ± 0.3% at 12 h. Pen mean molecular masses at these times, measured by high-performance liquid chromatography, were 160 ± 44 and 129 ± 23 kDa, respectively. In lung lymph, Pen concentrations were 0.8 ± 0.6% postinfusion and 0.7 ± 0.2% at 12 h, with mean molecular masses of 125 ± 44 and 112 ± 18 kDa, respectively. In soft tissue lymph Pen was nearly undetectable postinfusion, but at 12 h concentrations averaged 0.3 ± 0.2% with a mean molecular mass of 80 ± 10 kDa. The osmotic effectiveness of Pen may be related to its molecular mass, which was large enough to restrict filtration so that the plasma-to-lung lymph osmotic pressure gradient widened. Pen remained effective in the circulation for at least 24 h.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume265
Issue number5 34-5
StatePublished - 1993
Externally publishedYes

Fingerprint

Hydroxyethyl Starch Derivatives
Sheep
Lymph
Lung
Plasmapheresis
Osmotic Pressure
Plasma Substitutes
Pulmonary Wedge Pressure
Starch
Arterial Pressure
High Pressure Liquid Chromatography

Keywords

  • lymph flow
  • microvascular filtration
  • plasma osmotic pressure
  • plasma protein depletion
  • plasma volume expanders

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Effects of intravenous pentafraction on lung and soft tissue liquid exchange in hypoproteinemic sheep. / Conhaim, R. L.; Rosenfeld, D. J.; Schreiber, Martin; Baaske, D. M.; Harms, B. A.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 265, No. 5 34-5, 1993.

Research output: Contribution to journalArticle

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