Effects of intravenous pentafraction on lung and soft tissue liquid exchange in hypoproteinemic sheep

R. L. Conhaim, D. J. Rosenfeld, M. A. Schreiber, D. M. Baaske, B. A. Harms

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Effects of infusing pentafraction (Pen), a synthetic hydroxyethyl starch plasma volume expander, on lung and soft tissue lymph flux were compared in nonanesthetized sheep that were protein depleted by batch plasmapheresis. Pen (5%) was infused to raise pulmonary arterial wedge pressure by 5 mmHg for 2 h (1.8 ± 0.3 l). Pen raised plasma osmotic pressure from plasmapheresis baseline (10.7 ± 2.2 mmHg; preplasmapheresis baseline, 19.6 ± 0.6 mmHg) to 18.6 ± 2.4 mmHg. After Pen, lung lymph flows peaked at 3.9 ± 2.0 times a preplasmapheresis baseline value of 1.0 (plasmapheresis baseline, 2.7 ± 0.7), but soft tissue lymph flows rose insignificantly. Plasma Pen concentrations were 2.3 ± 1.0% postinfusion and 1.6 ± 0.3% at 12 h. Pen mean molecular masses at these times, measured by high-performance liquid chromatography, were 160 ± 44 and 129 ± 23 kDa, respectively. In lung lymph, Pen concentrations were 0.8 ± 0.6% postinfusion and 0.7 ± 0.2% at 12 h, with mean molecular masses of 125 ± 44 and 112 ± 18 kDa, respectively. In soft tissue lymph Pen was nearly undetectable postinfusion, but at 12 h concentrations averaged 0.3 ± 0.2% with a mean molecular mass of 80 ± 10 kDa. The osmotic effectiveness of Pen may be related to its molecular mass, which was large enough to restrict filtration so that the plasma-to-lung lymph osmotic pressure gradient widened. Pen remained effective in the circulation for at least 24 h.

Original languageEnglish (US)
Pages (from-to)H1536-H1543
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume265
Issue number5 34-5
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • lymph flow
  • microvascular filtration
  • plasma osmotic pressure
  • plasma protein depletion
  • plasma volume expanders

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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