Effects of intratympanic gentamicin on vestibular afferents and hair cells in the chinchilla

Timo P. Hirvonen, Lloyd B. Minor, Timothy Hullar, Joan P. Carey

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Gentamicin is toxic to vestibular hair cells, but its effects on vestibular afferents have not been defined. We treated anesthetized chinchillas with one injection of gentamicin (26.7 mg/ml) into the middle ear and made extracellular recordings from afferents after 5-25 (early) or 90-115 days (late). The relative proportions of regular, intermediate, and irregular afferents did not change after treatment. The spontaneous firing rate of regular afferents was lower (P <0.001) on the treated side (early: 44.3 ± 16.3; late: 33.9 ± 13.2 spikes · s-1) than on the untreated side (54.9 ± 16.8 spikes · s-1). Spontaneous rates of irregular and intermediate afferents did not change. The majority of treated afferents did not measurably respond to tilt or rotation (82% in the early group, 76% in the late group). Those that did respond had abnormally low sensitivities (P <0.001). Treated canal units that responded to rotation had mean sensitivities only 5-7% of the values for untreated canal afferents. Treated otolith afferents had mean sensitivities 23-28% of the values for untreated otolith units. Sensitivity to externally applied galvanic currents was unaffected for all afferents. Intratympanic gentamicin treatment reduced the histological density of all hair cells by 57% (P = 0.04). The density of hair cells with calyx endings was reduced by 99% (P = 0.03), although some remaining hair cells had other features suggestive of type I morphology. Type II hair cell density was not significantly reduced. These findings suggest that a single intratympanic gentamicin injection causes partial damage and loss of vestibular hair cells, particularly type I hair cells or their calyceal afferent endings, does not damage the afferent spike initiation zones, and preserves enough hair cell synaptic activity to drive the spontaneous activity of vestibular afferents.

Original languageEnglish (US)
Pages (from-to)643-655
Number of pages13
JournalJournal of Neurophysiology
Volume93
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

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Vestibular Hair Cells
Chinchilla
Gentamicins
Otolithic Membrane
Cell Count
Poisons
Middle Ear
Injections
Therapeutics

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Effects of intratympanic gentamicin on vestibular afferents and hair cells in the chinchilla. / Hirvonen, Timo P.; Minor, Lloyd B.; Hullar, Timothy; Carey, Joan P.

In: Journal of Neurophysiology, Vol. 93, No. 2, 02.2005, p. 643-655.

Research output: Contribution to journalArticle

Hirvonen, Timo P. ; Minor, Lloyd B. ; Hullar, Timothy ; Carey, Joan P. / Effects of intratympanic gentamicin on vestibular afferents and hair cells in the chinchilla. In: Journal of Neurophysiology. 2005 ; Vol. 93, No. 2. pp. 643-655.
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