Effects of intraarterial thrombin in the swine model

M. E. Pfister, R. T. Andrews, Dusan Pavcnik, Barry Uchida, Josef Rosch

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

PURPOSE: To evaluate the effects of catheter-directed thrombin in the peripheral arterial circulation of swine. MATERIALS AND METHODS: Thrombin was injected into a single femoral artery in 20 domestic swine. Each of five animals from four dose groups received 50, 150, 250, or 1,000 U as a single dose. Bilateral femoral arterial flow was monitored for as long as 4 hours and evaluated relative to baseline and contralateral limb flow. Interval arteriographic results were evaluated by segmental patency and a numeric angiographic score. RESULTS: Mean baseline flow was 136 mL/min ± 44, with an internal arterial diameter of 3.4 mm ± 0.5. A transient increase in blood flow after thrombin administration was followed by diminished flow and thrombosis. These findings varied directly with dose and inversely with baseline flow. Angiographic and flow abnormalities generally improved with time and recovery was generally better in swine that received 50 or 1,000 U than in other groups. However, one animal that received 1,000 U (13.2 U/mL/min) developed stable, complete limb thrombosis. The degree of recovery varied with thrombin dose and thrombus location. At doses greater than 50 U (0.33 u/mL/min ± 0.05), abnormalities were commonly persistent. Animals receiving the 150-U dose (1.33 U/mL/min ± 0.41) had a higher incidence of persistent distal occlusion. Distal occlusions were less likely to resolve than proximal occlusions. CONCLUSIONS: The effect of intraarterial thrombin is directly related to dose and inversely related to baseline blood flow. In swine, a threshold for significant flow disruption and thrombosis exists above a dose of 50 U (0.33 U/mL/min ± 0.05). A threshold dose for irreversible occlusion may also exist. Although small amounts of thrombin in a high-flow vessel may not cause significant complication, administration into the arterial circulation should be avoided.

Original languageEnglish (US)
Pages (from-to)235-245
Number of pages11
JournalJournal of Vascular and Interventional Radiology
Volume12
Issue number2
StatePublished - 2001
Externally publishedYes

Fingerprint

Thrombin
Swine
Thrombosis
Extremities
Femoral Artery
Thigh
Catheters
Incidence

Keywords

  • Arterial
  • Arteries
  • Complications
  • Experimental
  • Extremities
  • Intervention al procedures
  • Thrombosis
  • Thrombosis

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Pfister, M. E., Andrews, R. T., Pavcnik, D., Uchida, B., & Rosch, J. (2001). Effects of intraarterial thrombin in the swine model. Journal of Vascular and Interventional Radiology, 12(2), 235-245.

Effects of intraarterial thrombin in the swine model. / Pfister, M. E.; Andrews, R. T.; Pavcnik, Dusan; Uchida, Barry; Rosch, Josef.

In: Journal of Vascular and Interventional Radiology, Vol. 12, No. 2, 2001, p. 235-245.

Research output: Contribution to journalArticle

Pfister, ME, Andrews, RT, Pavcnik, D, Uchida, B & Rosch, J 2001, 'Effects of intraarterial thrombin in the swine model', Journal of Vascular and Interventional Radiology, vol. 12, no. 2, pp. 235-245.
Pfister, M. E. ; Andrews, R. T. ; Pavcnik, Dusan ; Uchida, Barry ; Rosch, Josef. / Effects of intraarterial thrombin in the swine model. In: Journal of Vascular and Interventional Radiology. 2001 ; Vol. 12, No. 2. pp. 235-245.
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N2 - PURPOSE: To evaluate the effects of catheter-directed thrombin in the peripheral arterial circulation of swine. MATERIALS AND METHODS: Thrombin was injected into a single femoral artery in 20 domestic swine. Each of five animals from four dose groups received 50, 150, 250, or 1,000 U as a single dose. Bilateral femoral arterial flow was monitored for as long as 4 hours and evaluated relative to baseline and contralateral limb flow. Interval arteriographic results were evaluated by segmental patency and a numeric angiographic score. RESULTS: Mean baseline flow was 136 mL/min ± 44, with an internal arterial diameter of 3.4 mm ± 0.5. A transient increase in blood flow after thrombin administration was followed by diminished flow and thrombosis. These findings varied directly with dose and inversely with baseline flow. Angiographic and flow abnormalities generally improved with time and recovery was generally better in swine that received 50 or 1,000 U than in other groups. However, one animal that received 1,000 U (13.2 U/mL/min) developed stable, complete limb thrombosis. The degree of recovery varied with thrombin dose and thrombus location. At doses greater than 50 U (0.33 u/mL/min ± 0.05), abnormalities were commonly persistent. Animals receiving the 150-U dose (1.33 U/mL/min ± 0.41) had a higher incidence of persistent distal occlusion. Distal occlusions were less likely to resolve than proximal occlusions. CONCLUSIONS: The effect of intraarterial thrombin is directly related to dose and inversely related to baseline blood flow. In swine, a threshold for significant flow disruption and thrombosis exists above a dose of 50 U (0.33 U/mL/min ± 0.05). A threshold dose for irreversible occlusion may also exist. Although small amounts of thrombin in a high-flow vessel may not cause significant complication, administration into the arterial circulation should be avoided.

AB - PURPOSE: To evaluate the effects of catheter-directed thrombin in the peripheral arterial circulation of swine. MATERIALS AND METHODS: Thrombin was injected into a single femoral artery in 20 domestic swine. Each of five animals from four dose groups received 50, 150, 250, or 1,000 U as a single dose. Bilateral femoral arterial flow was monitored for as long as 4 hours and evaluated relative to baseline and contralateral limb flow. Interval arteriographic results were evaluated by segmental patency and a numeric angiographic score. RESULTS: Mean baseline flow was 136 mL/min ± 44, with an internal arterial diameter of 3.4 mm ± 0.5. A transient increase in blood flow after thrombin administration was followed by diminished flow and thrombosis. These findings varied directly with dose and inversely with baseline flow. Angiographic and flow abnormalities generally improved with time and recovery was generally better in swine that received 50 or 1,000 U than in other groups. However, one animal that received 1,000 U (13.2 U/mL/min) developed stable, complete limb thrombosis. The degree of recovery varied with thrombin dose and thrombus location. At doses greater than 50 U (0.33 u/mL/min ± 0.05), abnormalities were commonly persistent. Animals receiving the 150-U dose (1.33 U/mL/min ± 0.41) had a higher incidence of persistent distal occlusion. Distal occlusions were less likely to resolve than proximal occlusions. CONCLUSIONS: The effect of intraarterial thrombin is directly related to dose and inversely related to baseline blood flow. In swine, a threshold for significant flow disruption and thrombosis exists above a dose of 50 U (0.33 U/mL/min ± 0.05). A threshold dose for irreversible occlusion may also exist. Although small amounts of thrombin in a high-flow vessel may not cause significant complication, administration into the arterial circulation should be avoided.

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KW - Arteries

KW - Complications

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KW - Extremities

KW - Intervention al procedures

KW - Thrombosis

KW - Thrombosis

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