Effects of Insulin-Like Growth Factor-1 Gene Transfer on Myosin Heavy Chains in Denervated Rat Laryngeal Muscle

Paul Flint, Hideki Nakagawa, Akihiro Shiotani, Michael E. Coleman, Bert W. O'Malley

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objectives/Hypothesis: To determine whether the myotrophic activity of human insulin-like growth factor (hIGF)-1 promotes restoration of normal myosin heavy chain (MHC) composition after nerve injury, MHC composition was analyzed after hIGF-1 gene transfer in denervated rat laryngeal muscle. Study Design: Animal model to study effects of gene transfer on laryngeal paralysis. Methods: In anesthetized rats, the left recurrent and superior laryngeal nerves are cut and suture ligated. A midline thyrotomy is performed, and the thyroarytenoid muscle is injected with a polyvinyl-based formulation containing a muscle specific expression system and hIGF-1 DNA (treatment group) or saline (control group). After 30 days, animals were killed, and the thyroarytenoid muscle was removed and processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Densitometric measurements were obtained to determine composition of MHCs. Results: As previously described, MHC composition in denervated laryngeal muscle was characterized by a decrease in type IIB and IIL and up-regulation of IIA/IIX. Compared with controls, hIGF-1 treated animals demonstrated a significant increase in expression of type IIB and IIL and a significant decrease in expression of type IIA/X. Conclusions: These findings suggest that the myotrophic effect of hIGF-1 gene transfer results in normalization of MHC composition in denervated muscle, with suppression of type IIA/X MHC and promotion of type IIL expression.

Original languageEnglish (US)
Pages (from-to)368-371
Number of pages4
JournalLaryngoscope
Volume114
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

Fingerprint

Laryngeal Muscles
Myosin Heavy Chains
Somatomedins
Genes
Laryngeal Nerves
Polyvinyls
Recurrent Laryngeal Nerve
Vocal Cord Paralysis
Muscles
Human Activities
Sodium Dodecyl Sulfate
Sutures
Polyacrylamide Gel Electrophoresis
Up-Regulation
Animal Models
Control Groups
DNA
Wounds and Injuries

Keywords

  • Gene therapy
  • IGF-1
  • Laryngeal paralysis
  • Muscle
  • Myosin heavy chain

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Effects of Insulin-Like Growth Factor-1 Gene Transfer on Myosin Heavy Chains in Denervated Rat Laryngeal Muscle. / Flint, Paul; Nakagawa, Hideki; Shiotani, Akihiro; Coleman, Michael E.; O'Malley, Bert W.

In: Laryngoscope, Vol. 114, No. 2, 02.2004, p. 368-371.

Research output: Contribution to journalArticle

Flint, Paul ; Nakagawa, Hideki ; Shiotani, Akihiro ; Coleman, Michael E. ; O'Malley, Bert W. / Effects of Insulin-Like Growth Factor-1 Gene Transfer on Myosin Heavy Chains in Denervated Rat Laryngeal Muscle. In: Laryngoscope. 2004 ; Vol. 114, No. 2. pp. 368-371.
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