Effects of IL-4 and Fcγ receptor II engagement on Egr-1 expression during stimulation of B lymphocytes by membrane immunoglobulin crosslinking

Stephen J. Klaus, Nancy E. Phillips, David Parker

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Egr-1 is an immediate early gene that is rapidly upregulated in response to mitogenic signals induced by antigen receptor crosslinking on murine B lymphocytes. It has been shown that levels of Egr-1 expression are closely correlated with B cell proliferation in several models of B cell activation and tolerance. We compared the expression of Egr-1 during B cell stimulation with Fab′2 and IgG anti-immunoglobulin (anti-IG), since it is known that Fab′2 anti-Ig is mitogenic while IgG anti-Ig is not, owing to a dominant inhibitory effect of crosslinking the B cell Fcγ RII to membrane Ig. While mitogenic doses of Fab′2 anti-Ig induce large and rapid increases in Egr-1 expression, IgG anti-Ig results in smaller increases in Egr-1 mRNA, comparable to that seen with submitogenic concentrations of Fab′2 anti-Ig. However, the correlation between Egr-1 expression and B cell proliferation breaks down when IL-4 is added as a co-mitogen to induce B cell proliferation with IgG anti-Ig or submitogenic concentrations of Fab′2 anti-Ig. No corresponding increases in Egr-1 mRNA levels are observed when IL-4 is added. Therefore, IL-4 overcomes Fc receptor-mediated inhibition of B cell proliferation without affecting inhibition of Egr-1 mRNA induction, as demonstrated earlier for c-myc mRNA in this system.

Original languageEnglish (US)
Pages (from-to)1553-1558
Number of pages6
JournalMolecular Immunology
Volume30
Issue number16
DOIs
StatePublished - 1993
Externally publishedYes

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Interleukin-4 Receptors
Fc Receptors
Immunoglobulins
B-Lymphocytes
Membranes
Cell Proliferation
Interleukin-4
Messenger RNA
Antigen Receptors
Immediate-Early Genes
Mitogens
anti-IgG

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Effects of IL-4 and Fcγ receptor II engagement on Egr-1 expression during stimulation of B lymphocytes by membrane immunoglobulin crosslinking. / Klaus, Stephen J.; Phillips, Nancy E.; Parker, David.

In: Molecular Immunology, Vol. 30, No. 16, 1993, p. 1553-1558.

Research output: Contribution to journalArticle

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