TY - JOUR
T1 - Effects of hypoxia on cochlear blood flow in mice evaluated using Doppler optical microangiography
AU - Dziennis, Suzan
AU - Reif, Roberto
AU - Zhi, Zhongwei
AU - Nuttall, Alfred L.
AU - Wang, Ruikang K.
N1 - Funding Information:
This work was supported in part by research grants from the National Institutes of Health (R01DC01201, RO1DC00105, P30DC005983). The content is solely the responsibility of the authors and does not necessarily represent the official views of the grant-giving bodies.
PY - 2012/10
Y1 - 2012/10
N2 - Reduced cochlear blood flow (CoBF) is a main contributor to hearing loss. Studying CoBF has remained a challenge due to the lack of available tools. Doppler optical microangiography (DOMAG), a method to quantify single-vessel absolute blood flow, and laser Doppler flowmetry (LDF), a method for measuring the relative blood flow within a large volume of tissue, were used for determining the changes in CoBF due to systemic hypoxia in mice. DOMAG determined the change in blood flow in the apical turn (AT) with single-vessel resolution, while LDF averaged the change in the blood flow within a large volume of the cochlea (hemisphere with ∼1 to 1.5 mm radius). Hypoxia was induced by decreasing the concentration of oxygen-inspired gas, so that the oxygen saturation was reduced from >95% to ∼80%. DOMAG determined that during hypoxia the blood flow in two areas of the AT near and far from the helicotrema were increased and decreased, respectively. The LDF detected a decrease in blood flow within a larger volume of the cochlea (several turns averaged together). Therefore, the use of DOMAG as a tool for studying cochlear blood flow due to its ability to determine absolute flow values with single-vessel resolution was proposed. . 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).
AB - Reduced cochlear blood flow (CoBF) is a main contributor to hearing loss. Studying CoBF has remained a challenge due to the lack of available tools. Doppler optical microangiography (DOMAG), a method to quantify single-vessel absolute blood flow, and laser Doppler flowmetry (LDF), a method for measuring the relative blood flow within a large volume of tissue, were used for determining the changes in CoBF due to systemic hypoxia in mice. DOMAG determined the change in blood flow in the apical turn (AT) with single-vessel resolution, while LDF averaged the change in the blood flow within a large volume of the cochlea (hemisphere with ∼1 to 1.5 mm radius). Hypoxia was induced by decreasing the concentration of oxygen-inspired gas, so that the oxygen saturation was reduced from >95% to ∼80%. DOMAG determined that during hypoxia the blood flow in two areas of the AT near and far from the helicotrema were increased and decreased, respectively. The LDF detected a decrease in blood flow within a larger volume of the cochlea (several turns averaged together). Therefore, the use of DOMAG as a tool for studying cochlear blood flow due to its ability to determine absolute flow values with single-vessel resolution was proposed. . 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).
KW - Cochlear blood flow
KW - Doppler optical microangiography
KW - Hypoxia
UR - http://www.scopus.com/inward/record.url?scp=84876572264&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876572264&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.17.10.106003
DO - 10.1117/1.JBO.17.10.106003
M3 - Article
C2 - 23224002
AN - SCOPUS:84876572264
SN - 1083-3668
VL - 17
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 10
M1 - 106003
ER -