Effects of exposure to chronic placental insufficiency on the postnatal brain and retina in sheep

Jhodie R. Duncan, Megan L. Cock, Michelle Loeliger, Samantha Louey, Richard Harding, Sandra M. Rees

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Chronic placental insufficiency (CPI) has the potential to affect fetal brain development and to cause brain injury. Our aim was to determine the effects of exposure to CPI during late gestation on brain and retinal structure and brain neurotrophin expression 8 weeks after birth. Six fetal sheep were exposed to CPI, induced by umbilico-placental embolization, from 120 days of gestation until term (∼147 days) such that fetal arterial oxygen saturation (SaO2) was reduced by ∼50%. Nine untreated animals served as controls. During CPI, fetal arterial PO2, SaO2, pH, and growth were reduced (p <0.05); these animals remained small at 8 weeks after birth. Structural abnormalities were present in the brains and retinae of all CPI-exposed lambs. There was a reduction in retinal width and in the number of retinal tyrosine hydroxylase-immunoreactive dopaminergic amacrine cells (p <0.05). In the dorsal hippocampus the combined width of strata oriens and pyramidale was significantly reduced (p <0.05). In the cerebellum there was a significant reduction (p = 0.05) in cerebellar cross-sectional area, most notably in the inner granule cell layer, and a reduction (p <0.05) in immunoreactivity for the cytoskeletal protein neurofilament-200 in the white matter. Gliosis was present in either the cerebral white matter or cerebellum in all animals and degeneration was seen around blood vessels in 4/6 umbilico-placental embolization animals. There were reductions in brain-derived neurotrophic factor immunoreactivity in the hippocampus (p <0.05) and tyrosine kinase B immunoreactivity in the cerebellum (p <0.05). This study shows that late gestational CPI affects morphology and neurotrophin expression of the postnatal brain. These alterations in the brain can apparently persist from fetal life or become established after birth; some changes that were present in the fetus at term did not persist into postnatal life.

Original languageEnglish (US)
Pages (from-to)1131-1143
Number of pages13
JournalJournal of Neuropathology and Experimental Neurology
Volume63
Issue number11
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Placental Insufficiency
Retina
Sheep
Brain
Cerebellum
Nerve Growth Factors
Parturition
Hippocampus
Amacrine Cells
Pregnancy
Gliosis
Cytoskeletal Proteins
Brain-Derived Neurotrophic Factor
Tyrosine 3-Monooxygenase
Fetal Development
Protein-Tyrosine Kinases
Brain Injuries
Blood Vessels
Fetus
Oxygen

Keywords

  • Brain damage
  • Intrauterine growth restriction
  • Neurotrophic factors
  • Sheep

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Effects of exposure to chronic placental insufficiency on the postnatal brain and retina in sheep. / Duncan, Jhodie R.; Cock, Megan L.; Loeliger, Michelle; Louey, Samantha; Harding, Richard; Rees, Sandra M.

In: Journal of Neuropathology and Experimental Neurology, Vol. 63, No. 11, 11.2004, p. 1131-1143.

Research output: Contribution to journalArticle

Duncan, Jhodie R. ; Cock, Megan L. ; Loeliger, Michelle ; Louey, Samantha ; Harding, Richard ; Rees, Sandra M. / Effects of exposure to chronic placental insufficiency on the postnatal brain and retina in sheep. In: Journal of Neuropathology and Experimental Neurology. 2004 ; Vol. 63, No. 11. pp. 1131-1143.
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