Effects of estrogen on hypothalamic gonadotropin-releasing hormone release in castrated male rhesus macaques

K. Y. Francis Pau, Perry M. Gliessman, David Hess, Harold G. Spies

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    The evidence that hypothalamic gonadotropin-releasing hormone (GnRH) release increases during the estrogen-induced luteinizing hormone (LH) surge in castrated female macaques and that estrogen induces a similar LH surge in the male suggests that either sexual differentiation of GnRH secretion does not exist or that changes in brain GnRH are not critical for ovulation. We tested this hypothesis by using the push-pull perfusion (PPP) technique to monitor GnRH release in the mediobasal hypothalamus continuously from 10 h before to 50 h after estrogen injection in 9 castrated male rhesus macaques. Blood sampling and PPP were performed with monkeys freely moving in their own cage by using a specially designed swivel/tethering system. PPP samples were collected every 10 min and analyzed for GnRH concentration by radioimmunoassay. Blood samples were collected every hour and plasma LH was measured by bioassay. Estradiol benzoate (EB, 42 μg/kg, b. wt.) was subcutaneously injected after 10 h of initial PPP. The PPP was continued for 10 h after EB in 4 and 50 h after EB in 5 monkeys. Hypothalamic GnRH patterns were analyzed by the Pulsar algorithms. The results show that during the 10 h after EB, plasma LH declined rapidly, reaching low or non-detectable levels by 7-9 h, while hypothalamic GnRH releasing patterns did not change during this period (n = 9). In contrast, estrogen enhanced GnRH level and pulse amplitude, but not pulse frequency, several hours before mean peripheral plasma LH increased from suppressed values (n = 4). These data suggest that the immediate negative feedback effect of estrogen on LH release occurs mainly in the pituitary, and that estrogen subsequently stimulates hypothalamic release of GnRH in advance of the initial rise in plasma LH. The results further support the hypothesis that the gonadotropin-releasing mechanism in primates is functionally non-differentiated, and that increased release of hypothalamic GnRH is actively involved in regulating the estrogen-induced gonadotropin surge in both sexes.

    Original languageEnglish (US)
    Pages (from-to)70-75
    Number of pages6
    JournalBrain Research
    Volume459
    Issue number1
    DOIs
    StatePublished - Aug 30 1988

    Fingerprint

    Pituitary Hormone-Releasing Hormones
    Macaca mulatta
    Gonadotropin-Releasing Hormone
    Estrogens
    Luteinizing Hormone
    Perfusion
    Gonadotropins
    Haplorhini
    DEAE-Dextran
    Sex Differentiation
    Macaca
    Ovulation
    Biological Assay
    Primates
    Hypothalamus
    Radioimmunoassay

    Keywords

    • Estrogen
    • Gonadotropin-releasing hormone
    • Luteinizing hormone
    • Male primate
    • Sexual dimorphism

    ASJC Scopus subject areas

    • Developmental Biology
    • Molecular Biology
    • Clinical Neurology
    • Neuroscience(all)

    Cite this

    Effects of estrogen on hypothalamic gonadotropin-releasing hormone release in castrated male rhesus macaques. / Francis Pau, K. Y.; Gliessman, Perry M.; Hess, David; Spies, Harold G.

    In: Brain Research, Vol. 459, No. 1, 30.08.1988, p. 70-75.

    Research output: Contribution to journalArticle

    Francis Pau, K. Y. ; Gliessman, Perry M. ; Hess, David ; Spies, Harold G. / Effects of estrogen on hypothalamic gonadotropin-releasing hormone release in castrated male rhesus macaques. In: Brain Research. 1988 ; Vol. 459, No. 1. pp. 70-75.
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