Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats1

Andrzej Bartke, Paul C. Doherty, Richard W. Steger, William W. Morgan, Armando G. Amador, Damon C. Herbert, Theresa M. Siler-Khodr, M (Susan) Smith, Harold G. Klemcke, Wesley C. Hymer

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Chronic estrogen treatment can lead to development of prolactin (PRL) secreting pituitary tumors. We have tested the ability of diethylstilbestrol (DES) to produce persistent hyperprolactinemia (hyperPRL) in adult male rats and examined the effects of this treatment on hypothalamic-pituitary-testicular function, adenohypophyseal structure, copulatory behavior and fertility. Silastic capsules containing approximately 5 mg DES were subcutaneously implanted into adult male CDF®(F-344)/CrlBR rats and removed 15 or 20 weeks later. Extreme hyperPRL, as well as suppression of plasma LH and FSH levels, persisted after DES capsules were removed. In contrast, plasma testosterone levels increased rapidly after removal of DES capsules and reached normal levels within 4-6 weeks. Copulatory behavior was assessed on two occasions between 7 and 14 weeks after removal of the DES capsules and was found to be suppressed in DES-treated rats, as evidenced by significant increases in latencies to mount, to intromit and to ejaculate. Moreover, when the animals were placed with normal females, the interval until conception was significantly greater in DES-treated than in control males. In spite of these differences in copulatory behavior, 10 of 11 DES-treated males were fertile. At autopsy, 44 weeks after capsule implantation (i.e. 24 or 29 weeks after capsule (removal), DES-treated rats had marked enlargement of the anterior pituitary, increased weights of the lateral prostate and the adrenals, increased levels of testicular hCG-binding sites, reduced concentration ot dopamine and norepinephrine in the median eminence and increased concentration of LHRH in the preoptic area DES-treated and control rats did not differ with respect to relative weights of the testes, ventral prostate and seminal vesicles and testicular testosterone production in vitro in the presence and in the absence of hCG. Immunocytochemical studies ot the adenohypophysis in a separate group of similarly treated animals revealed marked increases in the total number of parenchymal cells and in the relative proportion of mammotrophs. We conclude that chronic exposure of adult male CDF (F-344) rats to DES resulted in persistent massive mammotroph hyperplasia and that the resulting 20- to 100-fold elevatioti of peripheral PRL levels in these animals had little, if any, suppressive effect on testicular function, in spite of the drastic reduction in peripheral LH levels.

Original languageEnglish (US)
Pages (from-to)126-135
Number of pages10
JournalNeuroendocrinology
Volume39
Issue number2
StatePublished - 1984
Externally publishedYes

Fingerprint

Hyperprolactinemia
Diethylstilbestrol
Estrogens
Capsules
Prolactin
Testosterone
Prostate
Weights and Measures
Median Eminence
Anterior Pituitary Gland
Preoptic Area
Aptitude
Seminal Vesicles
Pituitary Neoplasms
Gonadotropin-Releasing Hormone
Hyperplasia
Fertility
Testis
Autopsy
Dopamine

Keywords

  • Brain neurotransmitters
  • Copulatory behavior
  • Fertility
  • Gonadotropins
  • Hyperprolactinemia
  • Testosterone

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Bartke, A., Doherty, P. C., Steger, R. W., Morgan, W. W., Amador, A. G., Herbert, D. C., ... Hymer, W. C. (1984). Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats1. Neuroendocrinology, 39(2), 126-135.

Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats1. / Bartke, Andrzej; Doherty, Paul C.; Steger, Richard W.; Morgan, William W.; Amador, Armando G.; Herbert, Damon C.; Siler-Khodr, Theresa M.; Smith, M (Susan); Klemcke, Harold G.; Hymer, Wesley C.

In: Neuroendocrinology, Vol. 39, No. 2, 1984, p. 126-135.

Research output: Contribution to journalArticle

Bartke, A, Doherty, PC, Steger, RW, Morgan, WW, Amador, AG, Herbert, DC, Siler-Khodr, TM, Smith, MS, Klemcke, HG & Hymer, WC 1984, 'Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats1', Neuroendocrinology, vol. 39, no. 2, pp. 126-135.
Bartke A, Doherty PC, Steger RW, Morgan WW, Amador AG, Herbert DC et al. Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats1. Neuroendocrinology. 1984;39(2):126-135.
Bartke, Andrzej ; Doherty, Paul C. ; Steger, Richard W. ; Morgan, William W. ; Amador, Armando G. ; Herbert, Damon C. ; Siler-Khodr, Theresa M. ; Smith, M (Susan) ; Klemcke, Harold G. ; Hymer, Wesley C. / Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats1. In: Neuroendocrinology. 1984 ; Vol. 39, No. 2. pp. 126-135.
@article{d11b3d883cef48e98cc027745c9650fd,
title = "Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats1",
abstract = "Chronic estrogen treatment can lead to development of prolactin (PRL) secreting pituitary tumors. We have tested the ability of diethylstilbestrol (DES) to produce persistent hyperprolactinemia (hyperPRL) in adult male rats and examined the effects of this treatment on hypothalamic-pituitary-testicular function, adenohypophyseal structure, copulatory behavior and fertility. Silastic capsules containing approximately 5 mg DES were subcutaneously implanted into adult male CDF{\circledR}(F-344)/CrlBR rats and removed 15 or 20 weeks later. Extreme hyperPRL, as well as suppression of plasma LH and FSH levels, persisted after DES capsules were removed. In contrast, plasma testosterone levels increased rapidly after removal of DES capsules and reached normal levels within 4-6 weeks. Copulatory behavior was assessed on two occasions between 7 and 14 weeks after removal of the DES capsules and was found to be suppressed in DES-treated rats, as evidenced by significant increases in latencies to mount, to intromit and to ejaculate. Moreover, when the animals were placed with normal females, the interval until conception was significantly greater in DES-treated than in control males. In spite of these differences in copulatory behavior, 10 of 11 DES-treated males were fertile. At autopsy, 44 weeks after capsule implantation (i.e. 24 or 29 weeks after capsule (removal), DES-treated rats had marked enlargement of the anterior pituitary, increased weights of the lateral prostate and the adrenals, increased levels of testicular hCG-binding sites, reduced concentration ot dopamine and norepinephrine in the median eminence and increased concentration of LHRH in the preoptic area DES-treated and control rats did not differ with respect to relative weights of the testes, ventral prostate and seminal vesicles and testicular testosterone production in vitro in the presence and in the absence of hCG. Immunocytochemical studies ot the adenohypophysis in a separate group of similarly treated animals revealed marked increases in the total number of parenchymal cells and in the relative proportion of mammotrophs. We conclude that chronic exposure of adult male CDF (F-344) rats to DES resulted in persistent massive mammotroph hyperplasia and that the resulting 20- to 100-fold elevatioti of peripheral PRL levels in these animals had little, if any, suppressive effect on testicular function, in spite of the drastic reduction in peripheral LH levels.",
keywords = "Brain neurotransmitters, Copulatory behavior, Fertility, Gonadotropins, Hyperprolactinemia, Testosterone",
author = "Andrzej Bartke and Doherty, {Paul C.} and Steger, {Richard W.} and Morgan, {William W.} and Amador, {Armando G.} and Herbert, {Damon C.} and Siler-Khodr, {Theresa M.} and Smith, {M (Susan)} and Klemcke, {Harold G.} and Hymer, {Wesley C.}",
year = "1984",
language = "English (US)",
volume = "39",
pages = "126--135",
journal = "Neuroendocrinology",
issn = "0028-3835",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats1

AU - Bartke, Andrzej

AU - Doherty, Paul C.

AU - Steger, Richard W.

AU - Morgan, William W.

AU - Amador, Armando G.

AU - Herbert, Damon C.

AU - Siler-Khodr, Theresa M.

AU - Smith, M (Susan)

AU - Klemcke, Harold G.

AU - Hymer, Wesley C.

PY - 1984

Y1 - 1984

N2 - Chronic estrogen treatment can lead to development of prolactin (PRL) secreting pituitary tumors. We have tested the ability of diethylstilbestrol (DES) to produce persistent hyperprolactinemia (hyperPRL) in adult male rats and examined the effects of this treatment on hypothalamic-pituitary-testicular function, adenohypophyseal structure, copulatory behavior and fertility. Silastic capsules containing approximately 5 mg DES were subcutaneously implanted into adult male CDF®(F-344)/CrlBR rats and removed 15 or 20 weeks later. Extreme hyperPRL, as well as suppression of plasma LH and FSH levels, persisted after DES capsules were removed. In contrast, plasma testosterone levels increased rapidly after removal of DES capsules and reached normal levels within 4-6 weeks. Copulatory behavior was assessed on two occasions between 7 and 14 weeks after removal of the DES capsules and was found to be suppressed in DES-treated rats, as evidenced by significant increases in latencies to mount, to intromit and to ejaculate. Moreover, when the animals were placed with normal females, the interval until conception was significantly greater in DES-treated than in control males. In spite of these differences in copulatory behavior, 10 of 11 DES-treated males were fertile. At autopsy, 44 weeks after capsule implantation (i.e. 24 or 29 weeks after capsule (removal), DES-treated rats had marked enlargement of the anterior pituitary, increased weights of the lateral prostate and the adrenals, increased levels of testicular hCG-binding sites, reduced concentration ot dopamine and norepinephrine in the median eminence and increased concentration of LHRH in the preoptic area DES-treated and control rats did not differ with respect to relative weights of the testes, ventral prostate and seminal vesicles and testicular testosterone production in vitro in the presence and in the absence of hCG. Immunocytochemical studies ot the adenohypophysis in a separate group of similarly treated animals revealed marked increases in the total number of parenchymal cells and in the relative proportion of mammotrophs. We conclude that chronic exposure of adult male CDF (F-344) rats to DES resulted in persistent massive mammotroph hyperplasia and that the resulting 20- to 100-fold elevatioti of peripheral PRL levels in these animals had little, if any, suppressive effect on testicular function, in spite of the drastic reduction in peripheral LH levels.

AB - Chronic estrogen treatment can lead to development of prolactin (PRL) secreting pituitary tumors. We have tested the ability of diethylstilbestrol (DES) to produce persistent hyperprolactinemia (hyperPRL) in adult male rats and examined the effects of this treatment on hypothalamic-pituitary-testicular function, adenohypophyseal structure, copulatory behavior and fertility. Silastic capsules containing approximately 5 mg DES were subcutaneously implanted into adult male CDF®(F-344)/CrlBR rats and removed 15 or 20 weeks later. Extreme hyperPRL, as well as suppression of plasma LH and FSH levels, persisted after DES capsules were removed. In contrast, plasma testosterone levels increased rapidly after removal of DES capsules and reached normal levels within 4-6 weeks. Copulatory behavior was assessed on two occasions between 7 and 14 weeks after removal of the DES capsules and was found to be suppressed in DES-treated rats, as evidenced by significant increases in latencies to mount, to intromit and to ejaculate. Moreover, when the animals were placed with normal females, the interval until conception was significantly greater in DES-treated than in control males. In spite of these differences in copulatory behavior, 10 of 11 DES-treated males were fertile. At autopsy, 44 weeks after capsule implantation (i.e. 24 or 29 weeks after capsule (removal), DES-treated rats had marked enlargement of the anterior pituitary, increased weights of the lateral prostate and the adrenals, increased levels of testicular hCG-binding sites, reduced concentration ot dopamine and norepinephrine in the median eminence and increased concentration of LHRH in the preoptic area DES-treated and control rats did not differ with respect to relative weights of the testes, ventral prostate and seminal vesicles and testicular testosterone production in vitro in the presence and in the absence of hCG. Immunocytochemical studies ot the adenohypophysis in a separate group of similarly treated animals revealed marked increases in the total number of parenchymal cells and in the relative proportion of mammotrophs. We conclude that chronic exposure of adult male CDF (F-344) rats to DES resulted in persistent massive mammotroph hyperplasia and that the resulting 20- to 100-fold elevatioti of peripheral PRL levels in these animals had little, if any, suppressive effect on testicular function, in spite of the drastic reduction in peripheral LH levels.

KW - Brain neurotransmitters

KW - Copulatory behavior

KW - Fertility

KW - Gonadotropins

KW - Hyperprolactinemia

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=0021245561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021245561&partnerID=8YFLogxK

M3 - Article

VL - 39

SP - 126

EP - 135

JO - Neuroendocrinology

JF - Neuroendocrinology

SN - 0028-3835

IS - 2

ER -