Effects of eosinophils on nerve cell morphology and development

The role of reactive oxygen species and p38 MAP kinase

Paul J. Kingham, W. Graham McLean, Marie Therese Walsh, Allison Fryer, Gerald J. Gleich, Richard W. Costello

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The adhesion of eosinophils to nerve cells and the subsequent release of eosinophil products may contribute to the pathogenesis of conditions such as asthma and inflammatory bowel disease. In this study we have separately examined the consequences of eosinophil adhesion and degranulation for nerve cell morphology and development. Eosinophils induced neurite retraction of cultured guinea pig parasympathetic nerves and differentiated IMR32 cholinergic neuroblastoma cells. Inhibition of eosinophil adhesion to IMR32 cells attenuated this retraction. Eosinophil adhesion to IMR32 cells led to tyrosine phosphorylation of a number of nerve cell proteins, activation of p38 MAP kinase, and generation of neuronal reactive oxygen species (ROS). Inhibition of tyrosine kinases with genistein prevented both the generation of ROS in the nerve cells and neurite retraction. The p38 MAP kinase inhibitor SB-239063 prevented neurite retraction but had no effect on the induction of ROS. Thus eosinophils induced neurite retraction via two distinct pathways: by generation of tyrosine kinase-dependent ROS and by p38 MAP kinase. Eosinophils also prevented neurite outgrowth during differentiation of IMR32 cells. In contrast to their effect on neurite retraction, this effect was mimicked by medium containing products released from eosinophils and by eosinophil major basic protein. These results indicate that eosinophils modify the morphology of nerve cells by distinct mechanisms that involve adhesion and released proteins.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume285
Issue number4 29-4
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

p38 Mitogen-Activated Protein Kinases
Eosinophils
Reactive Oxygen Species
Neurons
Neurites
Protein-Tyrosine Kinases
Eosinophil Major Basic Protein
Genistein
Neuroblastoma
Inflammatory Bowel Diseases
Cholinergic Agents
Tyrosine
Cell Differentiation
Guinea Pigs
Proteins
Asthma
Phosphorylation

Keywords

  • Cell adhesion molecule
  • Cholinergic nerve
  • Inflammation
  • Major basic protein

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Effects of eosinophils on nerve cell morphology and development : The role of reactive oxygen species and p38 MAP kinase. / Kingham, Paul J.; McLean, W. Graham; Walsh, Marie Therese; Fryer, Allison; Gleich, Gerald J.; Costello, Richard W.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 285, No. 4 29-4, 01.10.2003.

Research output: Contribution to journalArticle

Kingham, Paul J. ; McLean, W. Graham ; Walsh, Marie Therese ; Fryer, Allison ; Gleich, Gerald J. ; Costello, Richard W. / Effects of eosinophils on nerve cell morphology and development : The role of reactive oxygen species and p38 MAP kinase. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2003 ; Vol. 285, No. 4 29-4.
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