Effects of different oocyte activation procedures on development and gene expression of porcine pre-implantation embryos

R. P. Cervera, M. A. Silvestre, N. Martí, E. García-Mengual, R. Moreno, M. Stojkovic

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Among the factors that affect the efficiency of somatic cell nuclear transfer (SCNT) in pigs, the activation protocol is the most variable among the current SCNT procedures. The aim of this study is focused on defining an efficient activation treatment of porcine oocytes. In Experiment 1, we studied the effects of nine different oocyte activation procedures (including chemical- and electrical-based treatments) on parthenogenetic embryo development. In Experiment 2, we studied the effect of the more efficient activation procedures on the gene expression profile of Oct4 and Igf2r in parthenogenetic blastocysts. In conclusion, ionomycin as a first calcium stimulus is not able to activate porcine oocytes efficiently in comparison with electric procedures. Electrical treatments with 6-DMAP significantly increased the level of Oct4 expression, whereas the single and double pulse treatments alone maintained the same profile as the IVF group.

Original languageEnglish (US)
Pages (from-to)e12-e20
JournalReproduction in Domestic Animals
Volume45
Issue number5
DOIs
StatePublished - Oct 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Endocrinology

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