Effects of dermal wounding on distal primary tumor immunobiology in mice

Leah M. Pyter, Daniel B. McKim, Yasmin Husain, Humberto Calero, Jonathan P. Godbout, John F. Sheridan, Phillip T. Marucha, Christopher G. Engeland

Research output: Research - peer-reviewArticle

Abstract

Background Before primary oral tumors are treated, various prophylactic procedures that require tissue repair are often necessary (e.g. biopsies, tooth extractions, radiation, and tracheotomies). Wound healing and tumor growth harness similar immune/inflammatory mechanisms. Our previous work indicates that tumors impair wound healing, although the extent to which tissue repair conversely influences tumor growth is poorly understood. Here, we test the hypothesis that dermal wound healing exacerbates primary tumor growth and influences tumor immunobiology. Materials and methods Female, immunocompetent mice were inoculated subcutaneously with murine oral cancer cells (AT-84) to induce flank tumors. Half of the mice received dermal excisional wounds (4 × 3.5 mm diameter) on their dorsum 16 days later, whereas the skin of controls remained intact. Tumor and blood tissues were harvested 1 and 5 days post wounding, and tumor myeloid cell populations and inflammatory gene expression were measured. Circulating myeloid cells, cytokines, and corticosterone were also quantified. Results Wounding increased tumor mass, early tumor infiltration of macrophages, and tumor inflammatory gene expression. While wounding attenuated tumor growth–induced increases in circulating myeloid cells, no effects of wounding on circulating cytokine/endocrine measures were observed. Conclusions These results indicate that modest skin immune/inflammatory processes can enhance distal tumor growth and alter innate tumor immunity. The implication for this work is that, in the presence of a tumor, the benefits of tissue-damaging procedures that occur clinically must be weighed against the potential consequences for tumor biology.

LanguageEnglish (US)
Pages328-335
Number of pages8
JournalJournal of Surgical Research
Volume221
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint

Skin
Neoplasms
Growth
Myeloid Cells
Wound Healing
Cytokines
Gene Expression
Tooth Extraction
Tracheotomy
Mouth Neoplasms
Corticosterone
Innate Immunity
Macrophages
Radiation
Biopsy
Wounds and Injuries
Population

Keywords

  • Cytokines
  • Myeloid cells
  • Oral cancer
  • Tissue repair
  • Trafficking

ASJC Scopus subject areas

  • Surgery

Cite this

Pyter, L. M., McKim, D. B., Husain, Y., Calero, H., Godbout, J. P., Sheridan, J. F., ... Engeland, C. G. (2018). Effects of dermal wounding on distal primary tumor immunobiology in mice. Journal of Surgical Research, 221, 328-335. DOI: 10.1016/j.jss.2017.09.016

Effects of dermal wounding on distal primary tumor immunobiology in mice. / Pyter, Leah M.; McKim, Daniel B.; Husain, Yasmin; Calero, Humberto; Godbout, Jonathan P.; Sheridan, John F.; Marucha, Phillip T.; Engeland, Christopher G.

In: Journal of Surgical Research, Vol. 221, 01.01.2018, p. 328-335.

Research output: Research - peer-reviewArticle

Pyter, LM, McKim, DB, Husain, Y, Calero, H, Godbout, JP, Sheridan, JF, Marucha, PT & Engeland, CG 2018, 'Effects of dermal wounding on distal primary tumor immunobiology in mice' Journal of Surgical Research, vol 221, pp. 328-335. DOI: 10.1016/j.jss.2017.09.016
Pyter LM, McKim DB, Husain Y, Calero H, Godbout JP, Sheridan JF et al. Effects of dermal wounding on distal primary tumor immunobiology in mice. Journal of Surgical Research. 2018 Jan 1;221:328-335. Available from, DOI: 10.1016/j.jss.2017.09.016
Pyter, Leah M. ; McKim, Daniel B. ; Husain, Yasmin ; Calero, Humberto ; Godbout, Jonathan P. ; Sheridan, John F. ; Marucha, Phillip T. ; Engeland, Christopher G./ Effects of dermal wounding on distal primary tumor immunobiology in mice. In: Journal of Surgical Research. 2018 ; Vol. 221. pp. 328-335
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