Effects of chronic hypoxia on cardiac function measured by pressure-volume catheter in fetal chickens

Sonnet Jonker, George Giraud, Herbert M. Espinoza, Erica N. Davis, Dane A. Crossley

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Hypoxia is a common component of many developmental insults and has been studied in earlystage chicken development. However, its impact on cardiac function and arterial-ventricular coupling in late-stage chickens is relatively unknown. To test the hypothesis that hypoxic incubation would reduce baseline cardiac function but protect the heart during acute hypoxia in late-stage chickens, white Leghorn eggs were incubated at 21% O2 or 15% O2. At 90% of incubation (19 days), hypoxic incubation caused growth restriction (-20%) and increased the LVto- body ratio (+41%). Left ventricular (LV) pressure-volume loops were measured in anesthetized chickens in normoxia and acute hypoxia (10% O2). Hypoxic incubation lowered the maximal rate of pressure generation (∆P/∆tMax; -22%) and output (-57%), whereas increasing end-systolic elastance (ELV; +31%) and arterial elastance (EA; +122%) at similar heart rates to normoxic incubation. Both hypoxic incubation and acute hypoxia lengthened the half-time of relaxation (τ; +24%). Acute hypoxia reduced heart rate (-8%) and increased end-diastolic pressure (+35%). Hearts were collected for mRNA analysis. Hypoxic incubation was marked by decreased mRNA expression of sarco(endo)plasmic reticulum Ca2+-ATPase 2, Na+/Ca2+ exchanger 1, phospholamban, and ryanodine receptor. In summary, hypoxic incubation reduces LV function in the late-stage chicken by slowing pressure generation and relaxation, which may be driven by altered intracellular excitation-contraction coupling. Cardiac efficiency is greatly reduced after hypoxic incubation. In both incubation groups acute hypoxia reduced diastolic function.

    Original languageEnglish (US)
    Pages (from-to)R680-R689
    JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
    Volume308
    Issue number8
    DOIs
    StatePublished - Apr 15 2015

    Fingerprint

    Chickens
    Catheters
    Pressure
    Heart Rate
    Excitation Contraction Coupling
    Egg White
    Reticulum
    Messenger RNA
    Ryanodine Receptor Calcium Release Channel
    Ventricular Function
    Calcium-Transporting ATPases
    Ventricular Pressure
    Left Ventricular Function
    Hypoxia
    Blood Pressure
    Growth

    Keywords

    • Animal model
    • Embryo
    • Heart development
    • Intrauterine growth restriction
    • Prenatal hypoxia

    ASJC Scopus subject areas

    • Physiology
    • Physiology (medical)

    Cite this

    Effects of chronic hypoxia on cardiac function measured by pressure-volume catheter in fetal chickens. / Jonker, Sonnet; Giraud, George; Espinoza, Herbert M.; Davis, Erica N.; Crossley, Dane A.

    In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 308, No. 8, 15.04.2015, p. R680-R689.

    Research output: Contribution to journalArticle

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    abstract = "Hypoxia is a common component of many developmental insults and has been studied in earlystage chicken development. However, its impact on cardiac function and arterial-ventricular coupling in late-stage chickens is relatively unknown. To test the hypothesis that hypoxic incubation would reduce baseline cardiac function but protect the heart during acute hypoxia in late-stage chickens, white Leghorn eggs were incubated at 21{\%} O2 or 15{\%} O2. At 90{\%} of incubation (19 days), hypoxic incubation caused growth restriction (-20{\%}) and increased the LVto- body ratio (+41{\%}). Left ventricular (LV) pressure-volume loops were measured in anesthetized chickens in normoxia and acute hypoxia (10{\%} O2). Hypoxic incubation lowered the maximal rate of pressure generation (∆P/∆tMax; -22{\%}) and output (-57{\%}), whereas increasing end-systolic elastance (ELV; +31{\%}) and arterial elastance (EA; +122{\%}) at similar heart rates to normoxic incubation. Both hypoxic incubation and acute hypoxia lengthened the half-time of relaxation (τ; +24{\%}). Acute hypoxia reduced heart rate (-8{\%}) and increased end-diastolic pressure (+35{\%}). Hearts were collected for mRNA analysis. Hypoxic incubation was marked by decreased mRNA expression of sarco(endo)plasmic reticulum Ca2+-ATPase 2, Na+/Ca2+ exchanger 1, phospholamban, and ryanodine receptor. In summary, hypoxic incubation reduces LV function in the late-stage chicken by slowing pressure generation and relaxation, which may be driven by altered intracellular excitation-contraction coupling. Cardiac efficiency is greatly reduced after hypoxic incubation. In both incubation groups acute hypoxia reduced diastolic function.",
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