Effects of an inhibitor of tripeptidyl peptidase II (Ala-Ala-Phe-chloromethylketone) and its combination with an inhibitor of the chymotrypsin-like activity of the proteasome (PSI) on apoptosis, cell cycle and proteasome activity in U937 cells

M. Bury, I. Mlynarczuk, E. Pleban, G. Hoser, J. Kawiak, Cezary Wojcik

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

AAF-AMC is not a specific TPP II substrate, since it is also hydrolyzed by purified proteasomes. Moreover, AAF-cmk, claimed to be a specific TPP II inhibitor, also inhibits the chymotrypsin-like activity of the proteasome. While AAF-cmk itself is mildly cytostatic to U-937 cells and induces cell cycle block in G1, its combination with PSI does not induce an increase in the cytostatic/cytotoxic effects. This suggests that TPP II is possibly less important for cell metabolism than it was previously believed and it is less probable that it can be able to fully compensate for the loss of the proteasome function.

Original languageEnglish (US)
Pages (from-to)131-132
Number of pages2
JournalFolia Histochemica et Cytobiologica
Volume39
Issue number2
StatePublished - 2001
Externally publishedYes

Fingerprint

U937 Cells
Chymotrypsin
Proteasome Endopeptidase Complex
Cell Cycle
Cytostatic Agents
Apoptosis
tripeptidyl-peptidase 2

Keywords

  • Apoptosis
  • Cell cycle
  • Proteasome
  • Tripeptidyl peptidase II
  • U937 cells
  • Ubiquitin

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

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abstract = "AAF-AMC is not a specific TPP II substrate, since it is also hydrolyzed by purified proteasomes. Moreover, AAF-cmk, claimed to be a specific TPP II inhibitor, also inhibits the chymotrypsin-like activity of the proteasome. While AAF-cmk itself is mildly cytostatic to U-937 cells and induces cell cycle block in G1, its combination with PSI does not induce an increase in the cytostatic/cytotoxic effects. This suggests that TPP II is possibly less important for cell metabolism than it was previously believed and it is less probable that it can be able to fully compensate for the loss of the proteasome function.",
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T1 - Effects of an inhibitor of tripeptidyl peptidase II (Ala-Ala-Phe-chloromethylketone) and its combination with an inhibitor of the chymotrypsin-like activity of the proteasome (PSI) on apoptosis, cell cycle and proteasome activity in U937 cells

AU - Bury, M.

AU - Mlynarczuk, I.

AU - Pleban, E.

AU - Hoser, G.

AU - Kawiak, J.

AU - Wojcik, Cezary

PY - 2001

Y1 - 2001

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KW - Apoptosis

KW - Cell cycle

KW - Proteasome

KW - Tripeptidyl peptidase II

KW - U937 cells

KW - Ubiquitin

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