Effects of age on clock gene expression in the rhesus macaque pituitary gland

Brandon D. Sitzmann, Dario R. Lemos, Mary Ann Ottinger, Henryk Urbanski

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Recent studies have shown that circadian clock genes are expressed in various peripheral tissues, raising the possibility that multiple clocks regulate circadian physiology. To study clock gene expression in the rhesus macaque pituitary gland we used gene microarray data and found that the pituitary glands of young and old adult males express several components of the circadian clock (Per1, Per2, Cry1, Bmal1, Clock, Rev-erbα and Csnk1e{open}). Semi-quantitative reverse-transcription polymerase chain reaction (sqRT-PCR) confirmed the presence of these core-clock genes and detected significant age-related differences in the expression of Per2 sqRT-PCR also showed differential expression of core-clock genes at two opposing time-points over the 24-h day, with greater expression of Per2 and Bmal1 (P

    Original languageEnglish (US)
    Pages (from-to)696-705
    Number of pages10
    JournalNeurobiology of Aging
    Volume31
    Issue number4
    DOIs
    StatePublished - Apr 2010

    Fingerprint

    Pituitary Gland
    Macaca mulatta
    Circadian Clocks
    Gene Expression
    Genes
    Reverse Transcription
    Polymerase Chain Reaction
    Young Adult

    Keywords

    • Age
    • Circadian
    • Pituitary
    • Rhesus macaque

    ASJC Scopus subject areas

    • Clinical Neurology
    • Neuroscience(all)
    • Aging
    • Developmental Biology
    • Geriatrics and Gerontology

    Cite this

    Effects of age on clock gene expression in the rhesus macaque pituitary gland. / Sitzmann, Brandon D.; Lemos, Dario R.; Ottinger, Mary Ann; Urbanski, Henryk.

    In: Neurobiology of Aging, Vol. 31, No. 4, 04.2010, p. 696-705.

    Research output: Contribution to journalArticle

    Sitzmann, Brandon D. ; Lemos, Dario R. ; Ottinger, Mary Ann ; Urbanski, Henryk. / Effects of age on clock gene expression in the rhesus macaque pituitary gland. In: Neurobiology of Aging. 2010 ; Vol. 31, No. 4. pp. 696-705.
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