Effector memory T cell responses are associated with protection of rhesus monkeys from mucosal simian immunodeficiency virus challenge

Scott Hansen, Cassandra Vieville, Nathan Whizin, Lia Coyne-Johnson, Don C. Siess, Derek D. Drummond, Alfred W. Legasse, Michael Axthelm, Kelli Oswald, Charles M. Trubey, Michael Piatak, Jeffrey D. Lifson, Jay Nelson, Michael A. Jarvis, Louis Picker

Research output: Contribution to journalArticle

495 Citations (Scopus)

Abstract

The rapid onset of massive, systemic viral replication during primary HIV or simian immunodeficiency virus (SIV) infection and the immune evasion capabilities of these viruses pose fundamental problems for vaccines that depend upon initial viral replication to stimulate effector T cell expansion and differentiation. We hypothesized that vaccines designed to maintain differentiated effector memory T cell (TEM cell) responses at viral entry sites might improve efficacy by impairing viral replication at its earliest stage, and we have therefore developed SIV protein-encoding vectors based on rhesus cytomegalovirus (RhCMV), the prototypical inducer of life-long TEM cell responses. RhCMV vectors expressing SIV Gag, Rev-Tat-Nef and Env persistently infected rhesus macaques, regardless of preexisting RhCMV immunity, and primed and maintained robust, SIV-specific CD4+ and CD8+ TEM cell responses (characterized by coordinate tumor necrosis factor, interferon-γ and macrophage inflammatory protein-1β expression, cytotoxic degranulation and accumulation at extralymphoid sites) in the absence of neutralizing antibodies. Compared to control rhesus macaques, these vaccinated rhesus macaques showed increased resistance to acquisition of progressive SIVmac239 infection upon repeated limiting-dose intrarectal challenge, including four macaques who controlled rectal mucosal infection without progressive systemic dissemination. These data suggest a new paradigm for AIDS vaccine development - vaccines capable of generating and maintaining HIV-specific TEM cells might decrease the incidence of HIV acquisition after sexual exposure.

Original languageEnglish (US)
Pages (from-to)293-299
Number of pages7
JournalNature Medicine
Volume15
Issue number3
DOIs
StatePublished - Mar 2009

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Simian Immunodeficiency Virus
T-cells
Macaca mulatta
Viruses
Cytomegalovirus
T-Lymphocytes
Data storage equipment
Vaccines
Transmission electron microscopy
HIV
Immune Evasion
Macrophage Inflammatory Proteins
AIDS Vaccines
Macaca
Virus Diseases
Infection
Neutralizing Antibodies
Interferons
Cell Differentiation
Immunity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Effector memory T cell responses are associated with protection of rhesus monkeys from mucosal simian immunodeficiency virus challenge. / Hansen, Scott; Vieville, Cassandra; Whizin, Nathan; Coyne-Johnson, Lia; Siess, Don C.; Drummond, Derek D.; Legasse, Alfred W.; Axthelm, Michael; Oswald, Kelli; Trubey, Charles M.; Piatak, Michael; Lifson, Jeffrey D.; Nelson, Jay; Jarvis, Michael A.; Picker, Louis.

In: Nature Medicine, Vol. 15, No. 3, 03.2009, p. 293-299.

Research output: Contribution to journalArticle

Hansen, S, Vieville, C, Whizin, N, Coyne-Johnson, L, Siess, DC, Drummond, DD, Legasse, AW, Axthelm, M, Oswald, K, Trubey, CM, Piatak, M, Lifson, JD, Nelson, J, Jarvis, MA & Picker, L 2009, 'Effector memory T cell responses are associated with protection of rhesus monkeys from mucosal simian immunodeficiency virus challenge', Nature Medicine, vol. 15, no. 3, pp. 293-299. https://doi.org/10.1038/nm.1935
Hansen, Scott ; Vieville, Cassandra ; Whizin, Nathan ; Coyne-Johnson, Lia ; Siess, Don C. ; Drummond, Derek D. ; Legasse, Alfred W. ; Axthelm, Michael ; Oswald, Kelli ; Trubey, Charles M. ; Piatak, Michael ; Lifson, Jeffrey D. ; Nelson, Jay ; Jarvis, Michael A. ; Picker, Louis. / Effector memory T cell responses are associated with protection of rhesus monkeys from mucosal simian immunodeficiency virus challenge. In: Nature Medicine. 2009 ; Vol. 15, No. 3. pp. 293-299.
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