Effect of vasopressin administration and deficiency upon 3H-AVP binding sites in the CNS and periphery during development

P. Szot, K. M. Myers, D. M. Dorsa

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Arginine8-vasopressin (AVP, 40 μg/100 g b.wt., SC) was administered to male Long-Evans (LE) pups from day 1 to 7 of life and the pups were sacrificed on day 8 or 60. 3H-AVP binding was performed on membranes prepared from the liver, kidney, and septum. No significant changes were observed in the kidney or septum of animals 8 or 60 days old. However, the chronic AVP treatment did result in a significant increase in the density of 3H-AVP binding sites in the liver when compared to control day 8 pups (control 44 ± 2 vs. AVP 56 ± 3 fmol/mg protein), with no change in affinity. This effect was maintained into adulthood, as the day 60 AVP-treated LE rats also showed a significant increase in liver 3H-AVP binding sites compared to control (control 186 ± 9 vs. AVP 239 ± 14 fmol/mg protein), with no change in affinity. A comparison of 3H-AVP binding sites in 8-day-old LE, heterozygous Brattleboro (HET-BB), and homozygous Brattleboro rats (HOM-BB) was performed to assess the effect of complete (HOM-BB) and partial (HET-BB) VP deficiency on binding sites in the CNS and periphery. The liver again was the only tissue in which a change in 3H-AVP binding characteristics was noted. The HOM-BB rat (Bmax 144 ± 6 fmol/mg protein) displayed a significant increase in AVP binding sites from the LE rat (Bmax 100 ± 7 fmol/mg protein), while the 3H-AVP binding sites in the HET-BB rat liver (Bmax 69.8 ± 9 fmol/mg protein) were significantly lower than LE rats. Thus hepatic AVP receptors appear most sensitive to the presence or absence of vasopressin during the early postnatal period.

Original languageEnglish (US)
Pages (from-to)389-394
Number of pages6
JournalPeptides
Volume13
Issue number2
DOIs
StatePublished - Jan 1 1992

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Keywords

  • Brattleboro
  • Chronic AVP
  • Liver
  • Postnatal
  • Receptors

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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