Effect of vasoactive intestinal peptide on monkey prolactin secretion and cyclic AMP in culture: Interaction with estradiol and phenol red

    Research output: Contribution to journalArticle

    4 Scopus citations

    Abstract

    To test the hypothesis that estrogenic compounds may decrease the sensitivity of primate lactotropes to adenylate cyclase-mediated secretagogues, the effect of VIP on prolactin secretion and cAMP levels in serum-free monkey pituitary monolayer cultures was examined in the presence and absence of estradiol (E) and phenol red. In two experimental designs, E treatment was initiated on either the day after dispersion (split plate design) or 10 days after serum-free culture (whole plate design). VIP challenges (5, 50 and 500 nM) were administered for 4 h on days 10 and 20 of culture. There was a significant decrease in the maximal percent stimulation of prolactin by VIP when cultures were treated with E or phenol red. The average percent increase in prolactin at 5. 50 and 500 nM VIP equalled 23, 83 and 156% in the absence of phenol red, but equalled 14,43 and 112% when E was added to phenol red-free cultures. The percent stimulation by VIP in the presence of phenol red averaged 32. 62 and 97%, but addition of E with phenol red decreased the average stimulation to 26, 45 and 72%. respectively. Basal levels of cAMP were increased by E and phenol red. However, the maximal percent stimulation of cAMP by VIP was decreased in the presence of E and phenol red. In summary, E and phenol red act to decrease the maximal percent stimulation of prolactin secretion by VIP. This effect is reflected by a decrease in the maximal percent stimulation of intracellular cAMP.

    Original languageEnglish (US)
    Pages (from-to)576-585
    Number of pages10
    JournalNeuroendocrinology
    Volume51
    Issue number5
    DOIs
    StatePublished - Jan 1 1990

    Keywords

    • Cyclic AMP
    • Estradiol
    • Monkey
    • Phenol red
    • Pituitary, Prolactin
    • Vasoactive intestinal peptide

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism
    • Endocrinology
    • Endocrine and Autonomic Systems
    • Cellular and Molecular Neuroscience

    Fingerprint Dive into the research topics of 'Effect of vasoactive intestinal peptide on monkey prolactin secretion and cyclic AMP in culture: Interaction with estradiol and phenol red'. Together they form a unique fingerprint.

  • Cite this