Abstract
The effect of triethyllead (PbEt3) on entry of newly synthesized proteins into myelin was studied in an in vitro system of brain stem slices prepared from actively myelinating young rats. The slices were incubated 30 min with [3H]leucine then the labeling of proteins was halted by a chase with cold amino acid and the slices were incubated an additional 30 min with different concentrations of PbEt3. Proteins continued to appear in myelin after the chase and this process was inhibited by PbEt3; however, distinct differences among individual proteins were observed. Inhibition curves for proteolipid, intermediate, and Wolfgram proteins were of a decrementally increasing type and displayed highest inhibition values in the case of proteolipid proteins. On the other hand, deposition of basic proteins was unaffected by the toxin to the concentration of about 5 μm, resulting in a sigmoidal curve of inhibition. The present results indicate specificity of PbEt3 toward post-translational metabolism of integral myelin proteins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 89-94 |
| Number of pages | 6 |
| Journal | Experimental Neurology |
| Volume | 75 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1982 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Neuroscience(all)
- Neurology
Cite this
Effect of triethyllead on post-translational processing of myelin proteins. / Konat, G.; Offner, Halina.
In: Experimental Neurology, Vol. 75, No. 1, 1982, p. 89-94.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of triethyllead on post-translational processing of myelin proteins
AU - Konat, G.
AU - Offner, Halina
PY - 1982
Y1 - 1982
N2 - The effect of triethyllead (PbEt3) on entry of newly synthesized proteins into myelin was studied in an in vitro system of brain stem slices prepared from actively myelinating young rats. The slices were incubated 30 min with [3H]leucine then the labeling of proteins was halted by a chase with cold amino acid and the slices were incubated an additional 30 min with different concentrations of PbEt3. Proteins continued to appear in myelin after the chase and this process was inhibited by PbEt3; however, distinct differences among individual proteins were observed. Inhibition curves for proteolipid, intermediate, and Wolfgram proteins were of a decrementally increasing type and displayed highest inhibition values in the case of proteolipid proteins. On the other hand, deposition of basic proteins was unaffected by the toxin to the concentration of about 5 μm, resulting in a sigmoidal curve of inhibition. The present results indicate specificity of PbEt3 toward post-translational metabolism of integral myelin proteins.
AB - The effect of triethyllead (PbEt3) on entry of newly synthesized proteins into myelin was studied in an in vitro system of brain stem slices prepared from actively myelinating young rats. The slices were incubated 30 min with [3H]leucine then the labeling of proteins was halted by a chase with cold amino acid and the slices were incubated an additional 30 min with different concentrations of PbEt3. Proteins continued to appear in myelin after the chase and this process was inhibited by PbEt3; however, distinct differences among individual proteins were observed. Inhibition curves for proteolipid, intermediate, and Wolfgram proteins were of a decrementally increasing type and displayed highest inhibition values in the case of proteolipid proteins. On the other hand, deposition of basic proteins was unaffected by the toxin to the concentration of about 5 μm, resulting in a sigmoidal curve of inhibition. The present results indicate specificity of PbEt3 toward post-translational metabolism of integral myelin proteins.
UR - http://www.scopus.com/inward/record.url?scp=0020042604&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020042604&partnerID=8YFLogxK
U2 - 10.1016/0014-4886(82)90008-5
DO - 10.1016/0014-4886(82)90008-5
M3 - Article
C2 - 7060684
AN - SCOPUS:0020042604
VL - 75
SP - 89
EP - 94
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 1
ER -