Effect of training and growth hormone suppression on insulin-like growth factor I mRNA in young rats

S. Zanconato, D. Y. Moromisato, M. Y. Moromisato, J. Woods, J. A. Brasel, D. Leroith, Charles Roberts, D. M. Cooper

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The growth hormone (GH)-insulin-like growth factor I (IGF-I) axis plays a role in the adaptation to exercise training, but IGF-I gene expression in response to exercise training and GH suppression has not been studied. Twenty female rats underwent a 4-wk treadmill training program begun in the prepubertal period (day 14 of life). In 10 of the training rats, GH production was suppressed by anti-GH-releasing hormone antibodies (GH suppressed). IGF-I mRNA and protein levels were measured in liver and hindlimb skeletal muscle. GH suppression reduced IGF-I mRNA expression in the liver to a much greater extent than in the muscle. In the GH control rats, training induced significant increases in hepatic exon 1-derived IGF-I mRNA (mean increase 30%; P <0.05) and muscle exon 2-derived mRNA (mean increase 35%; P <0.05). In the GH-suppressed rats, only muscle exon 1-derived transcripts were significantly increased by training (55%; P <0.05) and this was associated with a significant increase in muscle IGF-I protein levels (P <0.05). We speculate that the anabolic response to training may involve both GH-dependent increases in IGF-I mRNA in the liver and GH-independent increases in the muscle.

Original languageEnglish (US)
Pages (from-to)2204-2209
Number of pages6
JournalJournal of Applied Physiology
Volume76
Issue number5
StatePublished - 1994
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor I
Growth Hormone
Messenger RNA
Muscles
Exons
Liver
Exercise
Growth Hormone-Releasing Hormone
Hindlimb
Skeletal Muscle
Proteins
Gene Expression
Education
Antibodies

Keywords

  • hindlimb muscle
  • insulin-like growth factor I protein level
  • liver

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Zanconato, S., Moromisato, D. Y., Moromisato, M. Y., Woods, J., Brasel, J. A., Leroith, D., ... Cooper, D. M. (1994). Effect of training and growth hormone suppression on insulin-like growth factor I mRNA in young rats. Journal of Applied Physiology, 76(5), 2204-2209.

Effect of training and growth hormone suppression on insulin-like growth factor I mRNA in young rats. / Zanconato, S.; Moromisato, D. Y.; Moromisato, M. Y.; Woods, J.; Brasel, J. A.; Leroith, D.; Roberts, Charles; Cooper, D. M.

In: Journal of Applied Physiology, Vol. 76, No. 5, 1994, p. 2204-2209.

Research output: Contribution to journalArticle

Zanconato, S, Moromisato, DY, Moromisato, MY, Woods, J, Brasel, JA, Leroith, D, Roberts, C & Cooper, DM 1994, 'Effect of training and growth hormone suppression on insulin-like growth factor I mRNA in young rats', Journal of Applied Physiology, vol. 76, no. 5, pp. 2204-2209.
Zanconato S, Moromisato DY, Moromisato MY, Woods J, Brasel JA, Leroith D et al. Effect of training and growth hormone suppression on insulin-like growth factor I mRNA in young rats. Journal of Applied Physiology. 1994;76(5):2204-2209.
Zanconato, S. ; Moromisato, D. Y. ; Moromisato, M. Y. ; Woods, J. ; Brasel, J. A. ; Leroith, D. ; Roberts, Charles ; Cooper, D. M. / Effect of training and growth hormone suppression on insulin-like growth factor I mRNA in young rats. In: Journal of Applied Physiology. 1994 ; Vol. 76, No. 5. pp. 2204-2209.
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