Hyperoxia has been shown to reduce inflammatory tissue responses (IR), but potential pulmonary Oxygen toxicity severely limits a ventilatory approach. The hypothesis was tested that topical application of normal saline (NS), supersaturated with oxygen (NS-O2) at 20,000 mmHg (27 bar) in a prototype delivery system developed in our laboratory, would reduce cutaneous IR after UV light exposure to the mouse ear. In 10 albino CD1 mice, after a 15 min. UV light exposure (100 mw/cm2 broad A, B, C) topical NS-O2 was applied to one ear and NS alone was applied to the contralateral ear for 4 hrs./day on five consecutive days. A separate group of mice (n=8) exposed to UV light with no topical therapy served as an additional control. IR was evaluated by serial measurement of ear thickness (T) with a spring loaded caliper. Results. As shown mean T associated with topical NS alone increased by maximum of 30%, 36 hrs. after UV exposure, an effect similar to that for control mice (p>0.05). In contrast, mean T associated with topical NS-O2 increased to a maximum of only 5% (p<0.0001), 36 hrs. after the exposure. Conclusion: Although additional parameters of the IR require evaluation, these preliminary results suggests that topical NS-O2 reduces tissue edema induced by UV light exposure in this model.
|Original language||English (US)|
|Journal||Journal of Investigative Medicine|
|State||Published - Jan 1 1996|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)