Effect of target-enriched multiplex polymerase chain reaction on patient outcomes and costs during the 2013–14 influenza season

A. Hassoun, M. D. Huff, E. Asis, K. Chahal, Amir Azarbal, S. Lu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The US Centers for Disease Control and Prevention recommends the initial use of rapid antigen influenza diagnostic test (RIDT) for the detection of influenza A (H1N1-09). Nasopharyngeal samples were tested from 246 patients for H1N1-09 using target-enriched multiplex polymerase chain reaction (TEM-PCR), of which 163 were additionally tested via RIDT. RIDTs had a sensitivity of 18.7% compared with TEM-PCR as the reference standard. Patients with false-negative RIDTs were withheld from 111 days of oseltamivir and 65 days of isolation. Patients negative for H1N1 via TEM-PCR had antiviral therapy immediately stopped, thereby evading 408 days of oseltamivir and 315 days of unnecessary isolation. This cost avoidance saved US$208,982.

Original languageEnglish (US)
Pages (from-to)366-370
Number of pages5
JournalJournal of Hospital Infection
Volume96
Issue number4
DOIs
StatePublished - Aug 1 2017
Externally publishedYes

Fingerprint

Multiplex Polymerase Chain Reaction
Human Influenza
Oseltamivir
Routine Diagnostic Tests
Costs and Cost Analysis
Antigens
Centers for Disease Control and Prevention (U.S.)
Antiviral Agents
Therapeutics

Keywords

  • Antimicrobial stewardship
  • H1N1
  • Hospitalization cost reduction
  • Influenza
  • Multiplex PCR
  • Oseltamivir

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Effect of target-enriched multiplex polymerase chain reaction on patient outcomes and costs during the 2013–14 influenza season. / Hassoun, A.; Huff, M. D.; Asis, E.; Chahal, K.; Azarbal, Amir; Lu, S.

In: Journal of Hospital Infection, Vol. 96, No. 4, 01.08.2017, p. 366-370.

Research output: Contribution to journalArticle

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