Effect of systemic lipopolysaccharide-induced inflammation on cytokine levels in the murine cochlea: A pilot study

Lourdes Quintanilla-Dieck, Barbara Larrain, Dennis Trune, Peter Steyger

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The objective was to detect changes in cytokine expression within cochleae in a murine model of systemic inflammation, with or without aminoglycoside exposure. Four groups of mice received 1 of the following: saline only, systemic bacterial lipopolysaccharides (LPS) for 6 hours to induce endotoxemia and inflammatory responses, systemic gentamicin for 3 hours, or both treatments. After exsanguination, pooled cochleae (4/group) were processed for enzyme-linked immunosorbent assay (ELISA) for 16 cytokines. Gentamicin alone did not change cochlear cytokine levels, while LPS (±gentamicin) substantially elevated cochlear expression of several cytokines, particularly interleukin-1α, interleukin-6, monocyte chemotactic protein-1, macrophage inflammatory protein-1α, and RANTES. Since cytokines increase blood-brain barrier permeability, we hypothesize that cytokine-enhanced permeability of the blood-labyrinth barrier (BLB) could potentiate aminoglycoside-induced ototoxicity. This pilot study demonstrated the feasibility of detecting cytokine expression in murine cochleae using ELISA and facilitates future studies investigating BLB permeability in animal models of systemic inflammation.

Original languageEnglish (US)
Pages (from-to)301-303
Number of pages3
JournalOtolaryngology - Head and Neck Surgery (United States)
Volume149
Issue number2
DOIs
StatePublished - Aug 2013

Fingerprint

Cochlea
Lipopolysaccharides
Cytokines
Inflammation
Gentamicins
Permeability
Aminoglycosides
Inner Ear
Enzyme-Linked Immunosorbent Assay
Exsanguination
Macrophage Inflammatory Proteins
Chemokine CCL5
Endotoxemia
Chemokine CCL2
Blood-Brain Barrier
Interleukin-1
Interleukin-6
Animal Models

Keywords

  • aminoglycosides
  • cochlear cytokines
  • ototoxicity
  • sepsis
  • systemic inflammation

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

Effect of systemic lipopolysaccharide-induced inflammation on cytokine levels in the murine cochlea : A pilot study. / Quintanilla-Dieck, Lourdes; Larrain, Barbara; Trune, Dennis; Steyger, Peter.

In: Otolaryngology - Head and Neck Surgery (United States), Vol. 149, No. 2, 08.2013, p. 301-303.

Research output: Contribution to journalArticle

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