Effect of Sustained Joint Loading on TMJ Disc Nutrient Environment

Y. Wu, S. E. Cisewski, M. C. Coombs, M. H. Brown, F. Wei, X. She, M. J. Kern, Y. M. Gonzalez, L. M. Gallo, V. Colombo, Laura Iwasaki, Jeffrey Nickel, H. Yao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The temporomandibular joint (TMJ) disc nutrient environment profoundly affects cell energy metabolism, proliferation, and biosynthesis. Due to technical challenges of in vivo measurements, the human TMJ disc extracellular nutrient environment under load, which depends on metabolic rates, solute diffusion, and disc morphometry, remains unknown. Therefore, the study objective was to predict the TMJ disc nutrient environment under loading conditions using combined experimental and computational modeling approaches. Specifically, glucose consumption and lactate production rates of porcine TMJ discs were measured under varying tissue culture conditions (n = 40 discs), and mechanical strain-dependent glucose and lactate diffusivities were measured using a custom diffusion chamber (n = 6 discs). TMJ anatomy and loading area were obtained from magnetic resonance imaging of healthy human volunteers (n = 11, male, 30 ± 9 y). Using experimentally determined nutrient metabolic rates, solute diffusivities, TMJ anatomy, and loading areas, subject-specific finite element (FE) models were developed to predict the 3-dimensional nutrient profiles in unloaded and loaded TMJ discs (unloaded, 0% strain, 20% strain). From the FE models, glucose, lactate, and oxygen concentration ranges for unloaded healthy human TMJ discs were 0.6 to 4.0 mM, 0.9 to 5.0 mM, and 0% to 6%, respectively, with steep gradients in the anterior and posterior bands. Sustained mechanical loading significantly reduced nutrient levels (P < 0.001), with a critical zone in which cells may die representing approximately 13.5% of the total disc volume. In conclusion, this study experimentally determined TMJ disc metabolic rates, solute diffusivities, and disc morphometry, and through subject-specific FE modeling, revealed critical interactions between mechanical loading and nutrient supply and metabolism for the in vivo human TMJ disc. The results suggest that TMJ disc homeostasis may be vulnerable to pathological loading (e.g., clenching, bruxism), which impedes nutrient supply. Given difficulties associated with direct in vivo measurements, this study provides a new approach to systematically investigate homeostatic and degenerative mechanisms associated with the TMJ disc.

Original languageEnglish (US)
Pages (from-to)888-895
Number of pages8
JournalJournal of dental research
Volume98
Issue number8
DOIs
StatePublished - Jul 1 2019

Fingerprint

Temporomandibular Joint Disc
Joints
Food
Lactic Acid
Temporomandibular Joint
Glucose
Anatomy
Bruxism
Energy Metabolism
Healthy Volunteers
Homeostasis
Swine

Keywords

  • cellular energy metabolism
  • compressive loading
  • disc nutrition
  • finite element analysis
  • solute diffusion
  • temporomandibular joint

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Wu, Y., Cisewski, S. E., Coombs, M. C., Brown, M. H., Wei, F., She, X., ... Yao, H. (2019). Effect of Sustained Joint Loading on TMJ Disc Nutrient Environment. Journal of dental research, 98(8), 888-895. https://doi.org/10.1177/0022034519851044

Effect of Sustained Joint Loading on TMJ Disc Nutrient Environment. / Wu, Y.; Cisewski, S. E.; Coombs, M. C.; Brown, M. H.; Wei, F.; She, X.; Kern, M. J.; Gonzalez, Y. M.; Gallo, L. M.; Colombo, V.; Iwasaki, Laura; Nickel, Jeffrey; Yao, H.

In: Journal of dental research, Vol. 98, No. 8, 01.07.2019, p. 888-895.

Research output: Contribution to journalArticle

Wu, Y, Cisewski, SE, Coombs, MC, Brown, MH, Wei, F, She, X, Kern, MJ, Gonzalez, YM, Gallo, LM, Colombo, V, Iwasaki, L, Nickel, J & Yao, H 2019, 'Effect of Sustained Joint Loading on TMJ Disc Nutrient Environment', Journal of dental research, vol. 98, no. 8, pp. 888-895. https://doi.org/10.1177/0022034519851044
Wu Y, Cisewski SE, Coombs MC, Brown MH, Wei F, She X et al. Effect of Sustained Joint Loading on TMJ Disc Nutrient Environment. Journal of dental research. 2019 Jul 1;98(8):888-895. https://doi.org/10.1177/0022034519851044
Wu, Y. ; Cisewski, S. E. ; Coombs, M. C. ; Brown, M. H. ; Wei, F. ; She, X. ; Kern, M. J. ; Gonzalez, Y. M. ; Gallo, L. M. ; Colombo, V. ; Iwasaki, Laura ; Nickel, Jeffrey ; Yao, H. / Effect of Sustained Joint Loading on TMJ Disc Nutrient Environment. In: Journal of dental research. 2019 ; Vol. 98, No. 8. pp. 888-895.
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abstract = "The temporomandibular joint (TMJ) disc nutrient environment profoundly affects cell energy metabolism, proliferation, and biosynthesis. Due to technical challenges of in vivo measurements, the human TMJ disc extracellular nutrient environment under load, which depends on metabolic rates, solute diffusion, and disc morphometry, remains unknown. Therefore, the study objective was to predict the TMJ disc nutrient environment under loading conditions using combined experimental and computational modeling approaches. Specifically, glucose consumption and lactate production rates of porcine TMJ discs were measured under varying tissue culture conditions (n = 40 discs), and mechanical strain-dependent glucose and lactate diffusivities were measured using a custom diffusion chamber (n = 6 discs). TMJ anatomy and loading area were obtained from magnetic resonance imaging of healthy human volunteers (n = 11, male, 30 ± 9 y). Using experimentally determined nutrient metabolic rates, solute diffusivities, TMJ anatomy, and loading areas, subject-specific finite element (FE) models were developed to predict the 3-dimensional nutrient profiles in unloaded and loaded TMJ discs (unloaded, 0{\%} strain, 20{\%} strain). From the FE models, glucose, lactate, and oxygen concentration ranges for unloaded healthy human TMJ discs were 0.6 to 4.0 mM, 0.9 to 5.0 mM, and 0{\%} to 6{\%}, respectively, with steep gradients in the anterior and posterior bands. Sustained mechanical loading significantly reduced nutrient levels (P < 0.001), with a critical zone in which cells may die representing approximately 13.5{\%} of the total disc volume. In conclusion, this study experimentally determined TMJ disc metabolic rates, solute diffusivities, and disc morphometry, and through subject-specific FE modeling, revealed critical interactions between mechanical loading and nutrient supply and metabolism for the in vivo human TMJ disc. The results suggest that TMJ disc homeostasis may be vulnerable to pathological loading (e.g., clenching, bruxism), which impedes nutrient supply. Given difficulties associated with direct in vivo measurements, this study provides a new approach to systematically investigate homeostatic and degenerative mechanisms associated with the TMJ disc.",
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AU - She, X.

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