Effect of serum on the isolated CNS myelin membrane

Gregory Konat, Halina Offner

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Rabbit brain myelin was incubated with rabbit serum, and changes in protein components were measured following re-isolation of the membrane by ultracentrifugation. Basic protein was substantially degraded by proteolytic enzymes present in serum. No breakdown of other major myelin proteins was observed. Some serum proteins became firmly associated with the myelin membrane during the incubation. Furthermore, the incubation of myelin with serum resulted in formation of denser myelin particles, which sedimented in 0.625 M sucrose. This process appears not to be related to the removal of basic protein from the membrane. The basic protein-digesting activity of human serum was comparable to that of rabbit serum, and no difference in the activity between sera from multiple sclerosis and control patients was observed. The possible relevance of these findings to demyelinating diseases is discussed.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalNeurochemistry International
Volume4
Issue number2-3
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Myelin Sheath
Membranes
Serum
Rabbits
Myelin Proteins
Proteins
Ultracentrifugation
Demyelinating Diseases
Human Activities
Multiple Sclerosis
Sucrose
Blood Proteins
Membrane Proteins
Peptide Hydrolases
Brain

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Effect of serum on the isolated CNS myelin membrane. / Konat, Gregory; Offner, Halina.

In: Neurochemistry International, Vol. 4, No. 2-3, 1982, p. 143-147.

Research output: Contribution to journalArticle

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