Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum: In vivo and in vitro studies

S. C. Kazmierczak, W. J. Castellani, F. Van Lente, E. D. Hodges, B. Udis

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3 Scopus citations


We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD;EC isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenzyme distribution between these two cell types. The ability of hemolysis to cause a 'flipped' LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.

Original languageEnglish (US)
Pages (from-to)1638-1641
Number of pages4
JournalClinical chemistry
Issue number9
StatePublished - Oct 19 1990


ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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