Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum: In vivo and in vitro studies

Steven (Steve) Kazmierczak, William J. Castellani, Frederick Van Lente, Edna D. Hodges, Barry Udls

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenyzme distribution between these two cell types. The ability of hemolysis to cause a "flipped" LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.

Original languageEnglish (US)
Pages (from-to)1638-1641
Number of pages4
JournalClinical Chemistry
Volume36
Issue number9
StatePublished - 1990
Externally publishedYes

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Reticulocytosis
L-Lactate Dehydrogenase
Isoenzymes
Reticulocytes
Hemolysis
Flow cytometry
Serum
Erythrocytes
Reticulocyte Count
Flow Cytometry
In Vitro Techniques

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum : In vivo and in vitro studies. / Kazmierczak, Steven (Steve); Castellani, William J.; Van Lente, Frederick; Hodges, Edna D.; Udls, Barry.

In: Clinical Chemistry, Vol. 36, No. 9, 1990, p. 1638-1641.

Research output: Contribution to journalArticle

Kazmierczak, Steven (Steve) ; Castellani, William J. ; Van Lente, Frederick ; Hodges, Edna D. ; Udls, Barry. / Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum : In vivo and in vitro studies. In: Clinical Chemistry. 1990 ; Vol. 36, No. 9. pp. 1638-1641.
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