Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum

In vivo and in vitro studies

Steven (Steve) Kazmierczak, William J. Castellani, Frederick Van Lente, Edna D. Hodges, Barry Udls

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenyzme distribution between these two cell types. The ability of hemolysis to cause a "flipped" LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.

Original languageEnglish (US)
Pages (from-to)1638-1641
Number of pages4
JournalClinical Chemistry
Volume36
Issue number9
StatePublished - 1990
Externally publishedYes

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Reticulocytosis
L-Lactate Dehydrogenase
Isoenzymes
Reticulocytes
Hemolysis
Flow cytometry
Serum
Erythrocytes
Reticulocyte Count
Flow Cytometry
In Vitro Techniques

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum : In vivo and in vitro studies. / Kazmierczak, Steven (Steve); Castellani, William J.; Van Lente, Frederick; Hodges, Edna D.; Udls, Barry.

In: Clinical Chemistry, Vol. 36, No. 9, 1990, p. 1638-1641.

Research output: Contribution to journalArticle

Kazmierczak, Steven (Steve) ; Castellani, William J. ; Van Lente, Frederick ; Hodges, Edna D. ; Udls, Barry. / Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum : In vivo and in vitro studies. In: Clinical Chemistry. 1990 ; Vol. 36, No. 9. pp. 1638-1641.
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