Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units

Jevgenia Zilberman-Rudenko, Frank Z. Zhao, Stephanie E. Reitsma, Annachiara Mitrugno, Jiaqing Pang, Joseph J. Shatzel, Beth Rick, Christina Tyrrell, Wohaib Hasan, Owen McCarty, Martin Schreiber

Research output: Contribution to journalArticle

Abstract

Platelet apheresis units are transfused into patients to mitigate or prevent bleeding. In a hospital, platelet apheresis units are transported from the transfusion service to the healthcare teams via two methods: a pneumatic tubing system (PTS) or ambulatory transport. Whether PTS transport affects the activity and utility of platelet apheresis units is unclear. We quantified the gravitational forces and transport time associated with PTS and ambulatory transport within our hospital. Washed platelets and supernatants were prepared from platelet apheresis units prior to transport as well as following ambulatory or PTS transport. For each group, we compared resting and agonist-induced platelet activity and platelet aggregate formation on collagen or von Willebrand factor (VWF) under shear, platelet VWF-receptor expression and VWF multimer levels. Subjection of platelet apheresis units to rapid acceleration/deceleration forces during PTS transport did not pre-activate platelets or their ability to activate in response to platelet agonists as compared to ambulatory transport. Platelets within platelet apheresis units transported via PTS retained their ability to adhere to surfaces of VWF and collagen under shear, although platelet aggregation on collagen and VWF was diminished as compared to ambulatory transport. VWF multimer levels and platelet GPIb receptor expression was unaffected by PTS transport as compared to ambulatory transport. Subjection of platelet apheresis units to PTS transport did not significantly affect the baseline or agonist-induced levels of platelet activation as compared to ambulatory transport. Our case study suggests that PTS transport may not significantly affect the hemostatic potential of platelets within platelet apheresis units.

Original languageEnglish (US)
Pages (from-to)515-527
Number of pages13
JournalCardiovascular Engineering and Technology
Volume9
Issue number3
DOIs
StatePublished - Sep 15 2018

Fingerprint

Blood Component Removal
Tubing
Platelets
Pneumatics
Blood Platelets
von Willebrand Factor
Collagen
Patient Care Team
Deceleration
Platelet Activation
Hemostatics

Keywords

  • Hemodynamics
  • Hemostasis
  • Platelet apheresis units
  • Platelet function
  • Pneumatic tubing system

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine

Cite this

Zilberman-Rudenko, J., Zhao, F. Z., Reitsma, S. E., Mitrugno, A., Pang, J., Shatzel, J. J., ... Schreiber, M. (2018). Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units. Cardiovascular Engineering and Technology, 9(3), 515-527. https://doi.org/10.1007/s13239-018-0361-2

Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units. / Zilberman-Rudenko, Jevgenia; Zhao, Frank Z.; Reitsma, Stephanie E.; Mitrugno, Annachiara; Pang, Jiaqing; Shatzel, Joseph J.; Rick, Beth; Tyrrell, Christina; Hasan, Wohaib; McCarty, Owen; Schreiber, Martin.

In: Cardiovascular Engineering and Technology, Vol. 9, No. 3, 15.09.2018, p. 515-527.

Research output: Contribution to journalArticle

Zilberman-Rudenko, J, Zhao, FZ, Reitsma, SE, Mitrugno, A, Pang, J, Shatzel, JJ, Rick, B, Tyrrell, C, Hasan, W, McCarty, O & Schreiber, M 2018, 'Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units', Cardiovascular Engineering and Technology, vol. 9, no. 3, pp. 515-527. https://doi.org/10.1007/s13239-018-0361-2
Zilberman-Rudenko J, Zhao FZ, Reitsma SE, Mitrugno A, Pang J, Shatzel JJ et al. Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units. Cardiovascular Engineering and Technology. 2018 Sep 15;9(3):515-527. https://doi.org/10.1007/s13239-018-0361-2
Zilberman-Rudenko, Jevgenia ; Zhao, Frank Z. ; Reitsma, Stephanie E. ; Mitrugno, Annachiara ; Pang, Jiaqing ; Shatzel, Joseph J. ; Rick, Beth ; Tyrrell, Christina ; Hasan, Wohaib ; McCarty, Owen ; Schreiber, Martin. / Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units. In: Cardiovascular Engineering and Technology. 2018 ; Vol. 9, No. 3. pp. 515-527.
@article{29cfce61e1d54972985b2386325bf91b,
title = "Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units",
abstract = "Platelet apheresis units are transfused into patients to mitigate or prevent bleeding. In a hospital, platelet apheresis units are transported from the transfusion service to the healthcare teams via two methods: a pneumatic tubing system (PTS) or ambulatory transport. Whether PTS transport affects the activity and utility of platelet apheresis units is unclear. We quantified the gravitational forces and transport time associated with PTS and ambulatory transport within our hospital. Washed platelets and supernatants were prepared from platelet apheresis units prior to transport as well as following ambulatory or PTS transport. For each group, we compared resting and agonist-induced platelet activity and platelet aggregate formation on collagen or von Willebrand factor (VWF) under shear, platelet VWF-receptor expression and VWF multimer levels. Subjection of platelet apheresis units to rapid acceleration/deceleration forces during PTS transport did not pre-activate platelets or their ability to activate in response to platelet agonists as compared to ambulatory transport. Platelets within platelet apheresis units transported via PTS retained their ability to adhere to surfaces of VWF and collagen under shear, although platelet aggregation on collagen and VWF was diminished as compared to ambulatory transport. VWF multimer levels and platelet GPIb receptor expression was unaffected by PTS transport as compared to ambulatory transport. Subjection of platelet apheresis units to PTS transport did not significantly affect the baseline or agonist-induced levels of platelet activation as compared to ambulatory transport. Our case study suggests that PTS transport may not significantly affect the hemostatic potential of platelets within platelet apheresis units.",
keywords = "Hemodynamics, Hemostasis, Platelet apheresis units, Platelet function, Pneumatic tubing system",
author = "Jevgenia Zilberman-Rudenko and Zhao, {Frank Z.} and Reitsma, {Stephanie E.} and Annachiara Mitrugno and Jiaqing Pang and Shatzel, {Joseph J.} and Beth Rick and Christina Tyrrell and Wohaib Hasan and Owen McCarty and Martin Schreiber",
year = "2018",
month = "9",
day = "15",
doi = "10.1007/s13239-018-0361-2",
language = "English (US)",
volume = "9",
pages = "515--527",
journal = "Cardiovascular Engineering and Technology",
issn = "1869-408X",
publisher = "Springer Publishing Company",
number = "3",

}

TY - JOUR

T1 - Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units

AU - Zilberman-Rudenko, Jevgenia

AU - Zhao, Frank Z.

AU - Reitsma, Stephanie E.

AU - Mitrugno, Annachiara

AU - Pang, Jiaqing

AU - Shatzel, Joseph J.

AU - Rick, Beth

AU - Tyrrell, Christina

AU - Hasan, Wohaib

AU - McCarty, Owen

AU - Schreiber, Martin

PY - 2018/9/15

Y1 - 2018/9/15

N2 - Platelet apheresis units are transfused into patients to mitigate or prevent bleeding. In a hospital, platelet apheresis units are transported from the transfusion service to the healthcare teams via two methods: a pneumatic tubing system (PTS) or ambulatory transport. Whether PTS transport affects the activity and utility of platelet apheresis units is unclear. We quantified the gravitational forces and transport time associated with PTS and ambulatory transport within our hospital. Washed platelets and supernatants were prepared from platelet apheresis units prior to transport as well as following ambulatory or PTS transport. For each group, we compared resting and agonist-induced platelet activity and platelet aggregate formation on collagen or von Willebrand factor (VWF) under shear, platelet VWF-receptor expression and VWF multimer levels. Subjection of platelet apheresis units to rapid acceleration/deceleration forces during PTS transport did not pre-activate platelets or their ability to activate in response to platelet agonists as compared to ambulatory transport. Platelets within platelet apheresis units transported via PTS retained their ability to adhere to surfaces of VWF and collagen under shear, although platelet aggregation on collagen and VWF was diminished as compared to ambulatory transport. VWF multimer levels and platelet GPIb receptor expression was unaffected by PTS transport as compared to ambulatory transport. Subjection of platelet apheresis units to PTS transport did not significantly affect the baseline or agonist-induced levels of platelet activation as compared to ambulatory transport. Our case study suggests that PTS transport may not significantly affect the hemostatic potential of platelets within platelet apheresis units.

AB - Platelet apheresis units are transfused into patients to mitigate or prevent bleeding. In a hospital, platelet apheresis units are transported from the transfusion service to the healthcare teams via two methods: a pneumatic tubing system (PTS) or ambulatory transport. Whether PTS transport affects the activity and utility of platelet apheresis units is unclear. We quantified the gravitational forces and transport time associated with PTS and ambulatory transport within our hospital. Washed platelets and supernatants were prepared from platelet apheresis units prior to transport as well as following ambulatory or PTS transport. For each group, we compared resting and agonist-induced platelet activity and platelet aggregate formation on collagen or von Willebrand factor (VWF) under shear, platelet VWF-receptor expression and VWF multimer levels. Subjection of platelet apheresis units to rapid acceleration/deceleration forces during PTS transport did not pre-activate platelets or their ability to activate in response to platelet agonists as compared to ambulatory transport. Platelets within platelet apheresis units transported via PTS retained their ability to adhere to surfaces of VWF and collagen under shear, although platelet aggregation on collagen and VWF was diminished as compared to ambulatory transport. VWF multimer levels and platelet GPIb receptor expression was unaffected by PTS transport as compared to ambulatory transport. Subjection of platelet apheresis units to PTS transport did not significantly affect the baseline or agonist-induced levels of platelet activation as compared to ambulatory transport. Our case study suggests that PTS transport may not significantly affect the hemostatic potential of platelets within platelet apheresis units.

KW - Hemodynamics

KW - Hemostasis

KW - Platelet apheresis units

KW - Platelet function

KW - Pneumatic tubing system

UR - http://www.scopus.com/inward/record.url?scp=85051492562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051492562&partnerID=8YFLogxK

U2 - 10.1007/s13239-018-0361-2

DO - 10.1007/s13239-018-0361-2

M3 - Article

C2 - 29785664

AN - SCOPUS:85051492562

VL - 9

SP - 515

EP - 527

JO - Cardiovascular Engineering and Technology

JF - Cardiovascular Engineering and Technology

SN - 1869-408X

IS - 3

ER -