Effect of pentobarbital on regional brain phospholipid synthesis

Reiko Natsuki, Robert Hitzemann, Barbara A. Hitzemann, Horace H. Loh

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Rats were given 45 mg/kg i.p. sodium pentobarbital 15 min prior to the intraventricular injection of 200 μCi [32P]phosphoric acid and 50 μCi [3H]glycerol. The animals were sacrificed 1 hr later, subcellular fractions were prepared from four subcortical brain regions and phospholipids were extracted. Pentobarbital significantly increased the ratio of [3H]- and [32P]-triphosphatidylinositol (TPI) to diphosphatidylinositol (DPI) in the microsomal but not synaptosomal fractions. The possible relationship of this change to nicotinic receptor activity is discussed. Pentobarbital specifically decreased 32Pi but not [3H]glycerol incorporation into synaptosomal phosphatidylinositol (PI). Thus, pentobarbital induced the opposite of the "neurotransmitter effect" on PI turnover. Pentobarbital either decreased or had no effect on the incorporation of 32Pi and [3H]glycerol into phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE).

Original languageEnglish (US)
Pages (from-to)2095-2100
Number of pages6
JournalBiochemical Pharmacology
Volume26
Issue number22
DOIs
Publication statusPublished - Nov 15 1977
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Pharmacology

Cite this